Open Access

Cantharidin alters the expression of genes associated with the NKG2D-associated immune response in TSGH-8301 human bladder carcinoma cells

  • Authors:
    • Jehn‑Hwa Kuo
    • An‑Cheng Huang
    • Jen‑Jyh Lin
    • Kuang‑Chi Lai
    • Rick Sai‑Chuen Wu
    • Jiun‑Long Yang
    • Bin‑Chuan Ji
    • Mei‑Due Yang
    • Yung‑Lin Chu
    • Jing‑Gung Chung
  • View Affiliations

  • Published online on: May 12, 2017     https://doi.org/10.3892/ol.2017.6168
  • Pages: 234-240
  • Copyright: © Kuo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cantharidin (CTD) is a natural toxin in beetles of the Mylabris genus (blister beetle), which has been revealed to induce cell death in various types of human cancer cells. However, to the best of our knowledge, no previous studies have investigated the effect of CTD on the expression of genes and their associated signaling pathways in human bladder carcinoma cells. In the present study, CTD‑induced cell morphological changes and apoptosis were observed using phase‑contrast microscopy and the terminal deoxynucleotidyl transferase dUTP nick end labeling assay, respectively, in TSGH‑8301 human bladder carcinoma cells. In addition, a complementary DNA microarray analysis demonstrated that CTD treatment led to a >2‑fold upregulation of 269 genes. For example, the DNA damage‑associated gene DNA‑damage‑inducible transcript 3 had a 4.75‑fold upregulation. Furthermore, another 286 genes were >2‑fold downregulated in response to CTD treatment. Matrix-remodeling associated 5, which is associated with cell migration and invasion, was downregulated 7.98-fold.
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July-2017
Volume 14 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Kuo JH, Huang AC, Lin JJ, Lai KC, Wu RS, Yang JL, Ji BC, Yang MD, Chu YL, Chung JG, Chung JG, et al: Cantharidin alters the expression of genes associated with the NKG2D-associated immune response in TSGH-8301 human bladder carcinoma cells. Oncol Lett 14: 234-240, 2017.
APA
Kuo, J., Huang, A., Lin, J., Lai, K., Wu, R.S., Yang, J. ... Chung, J. (2017). Cantharidin alters the expression of genes associated with the NKG2D-associated immune response in TSGH-8301 human bladder carcinoma cells. Oncology Letters, 14, 234-240. https://doi.org/10.3892/ol.2017.6168
MLA
Kuo, J., Huang, A., Lin, J., Lai, K., Wu, R. S., Yang, J., Ji, B., Yang, M., Chu, Y., Chung, J."Cantharidin alters the expression of genes associated with the NKG2D-associated immune response in TSGH-8301 human bladder carcinoma cells". Oncology Letters 14.1 (2017): 234-240.
Chicago
Kuo, J., Huang, A., Lin, J., Lai, K., Wu, R. S., Yang, J., Ji, B., Yang, M., Chu, Y., Chung, J."Cantharidin alters the expression of genes associated with the NKG2D-associated immune response in TSGH-8301 human bladder carcinoma cells". Oncology Letters 14, no. 1 (2017): 234-240. https://doi.org/10.3892/ol.2017.6168