WNT1-inducible signaling pathway protein-1 contributes to tumor progression and treatment failure in oral squamous cell carcinoma

Jung,Eun  Kyung; Kim,Sun‑Ae; Yoon,Tae  Mi; Lee,Kyung‑Hwa; Kim,Hee  Kyung; Lee,Dong  Hoon; Lee,Joon  Kyoo; Chung,Ik‑Joo; Joo,Young‑Eun; Lim,Sang  Chul

doi:10.3892/ol.2017.6313

WNT1-inducible signaling pathway protein-1 contributes to tumor progression and treatment failure in oral squamous cell carcinoma

Authors:
- Eun Kyung Jung
- Sun‑Ae Kim
- Tae Mi Yoon
- Kyung‑Hwa Lee
- Hee Kyung Kim
- Dong Hoon Lee
- Joon Kyoo Lee
- Ik‑Joo Chung
- Young‑Eun Joo
- Sang Chul Lim
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Affiliations: Department of Otorhinolaryngology‑Head and Neck Surgery, Chonnam National University Medical School, Gwangju, Chonnam 58128, Republic of Korea, Department of Pathology, Chonnam National University Medical School, Gwangju, Chonnam 58128, Republic of Korea, Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Chonnam 58128, Republic of Korea
Published online on: June 6, 2017 https://doi.org/10.3892/ol.2017.6313
Pages: 1719-1724

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Abstract

WNT1-inducible-signaling pathway protein-1 (WISP-1) belongs to the family of cysteine rich 61/connective tissue growth factor/nephroblastoma overexpressed matricellular proteins, which are involved in various biological processes, including cell adhesion, proliferation, differentiation, angiogenesis and carcinogenesis. In the present study, the expression of WISP‑1 was investigated, and its association with clinicopathological factors and prognosis in patients with oral squamous cell carcinoma (OSCC) was evaluated. Additionally, the role of WISP‑1 in invasion and apoptosis of human OSCC cells was evaluated. Immunoreactivity of WISP‑1 was increased in OSCC tissue compared with adjacent normal tissue samples. High expression of WISP‑1 protein was observed in 24/84 (28.57%) OSCC specimens. Additionally, high WISP‑1 expression was significantly associated with treatment failure (P=0.042). The 5‑year overall survival rate was 33% in patients with high WISP1 expression, and 66% in patients with low WISP‑1 expression. WISP‑1 expression in the human OSCC SCC‑1483 cell line was observed. Furthermore, WISP‑1 knockdown using small interfering (si)RNA significantly reduced cell invasion and induced apoptosis compared with control siRNA‑transfected cells. These findings suggested that WISP‑1 is associated with tumor progression and poor prognosis by increasing tumor cell invasion and inhibiting cell apoptosis in human OSCC.

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