The Chinese herb Prunella vulgaris promotes apoptosis in human well-differentiated thyroid carcinoma cells via the B-cell lymphoma-2/Bcl-2-associated X protein/caspase-3 signaling pathway

Yin,De‑Tao; Lei,Mengyuan; Xu,Jianhui; Li,Hongqiang; Wang,Yongfei; Liu,Zhen; Ma,Runsheng; Yu,Kun; Li,Xianghua

doi:10.3892/ol.2017.6317

The Chinese herb Prunella vulgaris promotes apoptosis in human well-differentiated thyroid carcinoma cells via the B-cell lymphoma-2/Bcl-2-associated X protein/caspase-3 signaling pathway

Authors:
- De‑Tao Yin
- Mengyuan Lei
- Jianhui Xu
- Hongqiang Li
- Yongfei Wang
- Zhen Liu
- Runsheng Ma
- Kun Yu
- Xianghua Li
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Affiliations: Department of Thyroid Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
Published online on: June 6, 2017 https://doi.org/10.3892/ol.2017.6317
Pages: 1309-1314
Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Prunella vulgaris (PV), a traditional Chinese herb, has been shown to be rich in bioactive chemicals and possess anti-proliferative and pro‑apoptotic effects on tumor cells. The effect of PV on human well‑differentiated thyroid carcinoma (WDTC), which accounts for the majority of common endocrine malignancies, remains to be elucidated. The present study aimed to investigate the function of PV on WDTC cell lines and apoptosis‑associated signaling pathway activity. Additional studies demonstrated that PV may induce apoptosis in WDTC TPC‑1 and FTC‑133 cell lines, using the Cell Counting Kit‑8 assay. Morphological changes of apoptotic cells were observed by Hoechst 33342 and acridine orange/ethidium bromide staining. In addition, ladder pattern of fragmented DNA was observed by DNA gel electrophoresis. It was also observed that PV significantly increased Bcl‑2‑associated X protein and caspase‑3 expression, and downregulated B‑cell lymphoma‑2 expression in TPC‑1 and FTC‑133 by reverse transcription‑quantitative polymerase chain reaction (P<0.05). Thus, the present results indicated that PV has the potential to be a future WDTC therapeutic agent.

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1	Yin D, Wu W, Li M, Wang QE, Li H, Wang Y, Tang Y and Xing M: DKK3 is a potential tumor suppressor gene in papillary thyroid carcinoma. Endocr Relat Cancer. 20:507–514. 2013. View Article : Google Scholar : PubMed/NCBI
2	Pacini F, Castagna MG, Brilli L and Pentheroudakis G; ESMO Guidelines Working Group, : Thyroid cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up. Ann Oncol. 23 Suppl 7:vii110–vii119. 2012. View Article : Google Scholar : PubMed/NCBI
3	Soares P, Lima J, Preto A, Castro P, Vinagre J, Celestino R, Couto JP, Prazeres H, Eloy C, Máximo V and Sobrinho-Simões M: Genetic alterations in poorly differentiated and undifferentiated thyroid carcinomas. Curr Genomics. 12:609–617. 2011. View Article : Google Scholar : PubMed/NCBI
4	Gruber JJ and Colevas AD: Differentiated thyroid cancer: Focus on emerging treatments for radioactive iodine-refractory patients. Oncologist. 20:113–126. 2015. View Article : Google Scholar : PubMed/NCBI
5	Kartal K, Onder S, Kosemehmetoglu K, Kilickap S, Tezel YG and Kaynaroglu V: Methylation status of TSHr in well-differentiated thyroid cancer by using cytologic material. BMC Cancer. 15:8242015. View Article : Google Scholar : PubMed/NCBI
6	Gunda V, Bucur O, Varnau J, Borre P Vanden, Bernasconi MJ, Khosravi-Far R and Parangi S: Blocks to thyroid cancer cell apoptosis can be overcome by inhibition of the MAPK and PI3K/AKT pathways. Cell Death Dis. 5:e11042014. View Article : Google Scholar : PubMed/NCBI
7	Li C, Huang Q, Fu X, Yue XJ, Liu RH and You LJ: Characterization, antioxidant and immunomodulatory activities of polysaccharides from Prunella vulgaris Linn. Int J Biol Macromol. 75:298–305. 2015. View Article : Google Scholar : PubMed/NCBI
8	Gu X, Li Y, Mu J and Zhang Y: Chemical constituents of Prunella vulgaris. J Environ Sci (China). 25 Suppl 1:S161–S163. 2013. View Article : Google Scholar : PubMed/NCBI
9	Guo Q and Chen Y: Textual research on original plant and dietotherapy history of Prunella vulgaris. Zhongguo Zhong Yao Za Zhi. 36:3057–3062. 2011.(In Chinese). PubMed/NCBI
10	Hwang YJ, Lee EJ, Kim HR and Hwang KA: NF-κB-targeted anti-inflammatory activity of Prunella vulgaris var. lilacina in macrophages RAW 264.7. Int J Mol Sci. 14:21489–21503. 2013. View Article : Google Scholar : PubMed/NCBI
11	Choi JH, Han EH, Hwang YP, Choi JM, Choi CY, Chung YC, Seo JK and Jeong HG: Suppression of PMA-induced tumor cell invasion and metastasis by aqueous extract isolated from Prunella vulgaris via the inhibition of NF-kappaB-dependent MMP-9 expression. Food Chem Toxicol. 48:564–571. 2010. View Article : Google Scholar : PubMed/NCBI
12	Collins NH, Lessey EC, DuSell CD, McDonnell DP, Fowler L, Palomino WA, Illera MJ, Yu X, Mo B, Houwing AM and Lessey BA: Characterization of antiestrogenic activity of the Chinese herb, prunella vulgaris, using in vitro and in vivo (Mouse Xenograft) models. Biol Reprod. 80:375–383. 2009. View Article : Google Scholar : PubMed/NCBI
13	Zhao AG, Li T, You SF, Zhao HL, Gu Y, Tang LD and Yang JK: Effects of Wei Chang An on expression of multiple genes in human gastric cancer grafted onto nude mice. World J Gastroenterol. 14:693–700. 2008. View Article : Google Scholar : PubMed/NCBI
14	Zhang KJ, Zhang MZ, Wang QD and Liu WL: The experimental research about the effect of Prunella vulgaris L. on Raji cells growth and expression of apoptosis related protein. Zhong Yao Cai. 29:1207–1210. 2006.(In Chinese). PubMed/NCBI
15	Feng L, Jia X, Zhu M, Chen Y and Shi F: Chemoprevention by Prunella vulgaris L. extract of non-small cell lung cancer via promoting apoptosis and regulating the cell cycle. Asian Pac J Cancer Prev. 11:1355–1358. 2010.PubMed/NCBI
16	Vethakanraj HS, Babu TA, Sudarsanan GB, Duraisamy PK and Kumar S Ashok: Targeting ceramide metabolic pathway induces apoptosis in human breast cancer cell lines. Biochem Biophys Res Commun. 464:833–839. 2015. View Article : Google Scholar : PubMed/NCBI
17	Leibowitz B and Yu J: Mitochondrial signaling in cell death via the Bcl-2 family. Cancer Biol Ther. 9:417–422. 2010. View Article : Google Scholar : PubMed/NCBI
18	Hwang SM, Lee YJ, Lee YP, Yoon JJ, Lee SM, Cha JD, Choi KM, Kang DG and Lee HS: Anti-proliferative effect of an aqueous extract of Prunella vulgaris in vascular smooth muscle cells. Evid Based Complement Alternat Med. 2013:9364632013. View Article : Google Scholar : PubMed/NCBI
19	Kim HI, Quan FS, Kim JE, Lee NR, Kim HJ, Jo SJ, Lee CM, Jang DS and Inn KS: Inhibition of estrogen signaling through depletion of estrogen receptor alpha by ursolic acid and betulinic acid from Prunella vulgaris var. lilacina. Biochem Biophys Res Commun. 451:282–287. 2014. View Article : Google Scholar : PubMed/NCBI
20	Dhivya R, Jaividhya P, Riyasdeen A, Palaniandavar M, Mathan G and Akbarsha MA: In vitro antiproliferative and apoptosis-inducing properties of a mononuclear copper (II) complex with dppz ligand, in two genotypically different breast cancer cell lines. Biometals. 28:929–943. 2015. View Article : Google Scholar : PubMed/NCBI
21	Schmid I, Uittenbogaart C and Jamieson BD: Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D. Nat Protoc. 2:187–190. 2007. View Article : Google Scholar : PubMed/NCBI
22	Ahamad MS, Siddiqui S, Jafri A, Ahmad S, Afzal M and Arshad M: Induction of apoptosis and antiproliferative activity of naringenin in human epidermoid carcinoma cell through ROS generation and cell cycle arrest. PLos One. 9:e1100032014. View Article : Google Scholar : PubMed/NCBI
23	Zhou WJ, Wang S, Hu Z, Zhou ZY and Song CJ: Angelica sinensis polysaccharides promotes apoptosis in human breast cancer cells via CREB-regulated caspase-3 activation. Biochem Biophys Res Commun. 467:562–569. 2015. View Article : Google Scholar : PubMed/NCBI
24	Hwang KT, Woo JW, Shin HC, Kim HS, Ahn SK, Moon HG, Han W, Park IA and Noh DY: Prognostic influence of BCL2 expression in breast cancer. Int J Cancer. 131:E1109–E1119. 2012. View Article : Google Scholar : PubMed/NCBI
25	Adams JM and Cory S: Bcl-2-regulated apoptosis: Mechanism and therapeutic potential. Curr Opin Immunol. 19:488–496. 2007. View Article : Google Scholar : PubMed/NCBI
26	Sabine VS, Faratian D, Kirkegaard-Clausen T and Bartlett JM: Validation of activated caspase-3 antibody staining as a marker of apoptosis in breast cancer. Histopathology. 60:369–371. 2012. View Article : Google Scholar : PubMed/NCBI