Open Access

Poly (ADP-ribose) polymerase-1 inhibition decreases proliferation through G2/M arrest in esophageal squamous cell carcinoma

  • Authors:
    • Masaaki Yamamoto
    • Makoto Yamasaki
    • Yukiko Tsukao
    • Koji Tanaka
    • Yasuhiro Miyazaki
    • Tomoki Makino
    • Tsuyoshi Takahashi
    • Yukinori Kurokawa
    • Kiyokazu Nakajima
    • Shuji Takiguchi
    • Masaki Mori
    • Yuichiro Doki
  • View Affiliations

  • Published online on: June 7, 2017     https://doi.org/10.3892/ol.2017.6334
  • Pages: 1581-1587
  • Copyright: © Yamamoto et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Poly (ADP-ribose) polymerase-1 (PARP1) plays a vital role in DNA repair and is expected to be an effective target in various malignancies. The aim of the present study was to investigate the clinical and biological significance of PARP1 expression in esophageal squamous cell carcinoma (ESCC). Immunohistochemical (IHC) staining was used to examine the association between PARP1 expression and the clinicopathological features of 86 patients with ESCC. The antitumor effect of small interfering RNA against PARP1 (siPARP1) was examined in a proliferation assay, and the mechanisms of this effect were investigated using western blot analysis and cell cycle assays. Cox multivariate analysis revealed that high expression of PARP1 in IHC staining was a statistically significant independent prognostic factor of poor overall survival (OS). The adjusted hazard ratio for OS in the group with high expression of PARP1 was 2.39 (95% confidence interval, 1.29‑4.44; P=0.0051). In vitro assays showed that siPARP1 significantly decreased proliferation through G2/M arrest. Furthermore, western blot analysis showed that PARP1 was associated with the ataxia telangiectasia mutated-checkpoint kinase 2‑cell division control 25c pathway. The present study suggests that PARP1 expression has a critical role in ESCC progression, and may be a clinical therapeutic target.
View Figures
View References

Related Articles

Journal Cover

August-2017
Volume 14 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yamamoto M, Yamasaki M, Tsukao Y, Tanaka K, Miyazaki Y, Makino T, Takahashi T, Kurokawa Y, Nakajima K, Takiguchi S, Takiguchi S, et al: Poly (ADP-ribose) polymerase-1 inhibition decreases proliferation through G2/M arrest in esophageal squamous cell carcinoma. Oncol Lett 14: 1581-1587, 2017.
APA
Yamamoto, M., Yamasaki, M., Tsukao, Y., Tanaka, K., Miyazaki, Y., Makino, T. ... Doki, Y. (2017). Poly (ADP-ribose) polymerase-1 inhibition decreases proliferation through G2/M arrest in esophageal squamous cell carcinoma. Oncology Letters, 14, 1581-1587. https://doi.org/10.3892/ol.2017.6334
MLA
Yamamoto, M., Yamasaki, M., Tsukao, Y., Tanaka, K., Miyazaki, Y., Makino, T., Takahashi, T., Kurokawa, Y., Nakajima, K., Takiguchi, S., Mori, M., Doki, Y."Poly (ADP-ribose) polymerase-1 inhibition decreases proliferation through G2/M arrest in esophageal squamous cell carcinoma". Oncology Letters 14.2 (2017): 1581-1587.
Chicago
Yamamoto, M., Yamasaki, M., Tsukao, Y., Tanaka, K., Miyazaki, Y., Makino, T., Takahashi, T., Kurokawa, Y., Nakajima, K., Takiguchi, S., Mori, M., Doki, Y."Poly (ADP-ribose) polymerase-1 inhibition decreases proliferation through G2/M arrest in esophageal squamous cell carcinoma". Oncology Letters 14, no. 2 (2017): 1581-1587. https://doi.org/10.3892/ol.2017.6334