MicroRNA-150 functions as an antioncogenic regulator in osteosarcoma

  • Authors:
    • Jin Xu
    • Zengliang Wang
    • Zhichao Liao
    • Dong Dai
    • Xinlong Ma
  • View Affiliations

  • Published online on: June 16, 2017     https://doi.org/10.3892/ol.2017.6393
  • Pages: 2483-2490
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Abstract

Numerous studies have demonstrated that microRNAs (miRs) are involved in several physiological and pathological processes, and participate in cancer initiation and progression. The abnormal expression of miR‑150 has been reported in numerous types of human cancer. However, at present there are no studies of miR‑150 in osteosarcoma (OS). Reverse transcription‑quantitative polymerase chain reaction was performed to measure miR‑150 expression levels in OS tissues and cell lines. Subsequent to transfection with miR‑150 mimics or zinc finger E‑box binding homeobox 1 (ZEB1) small interfering RNA, an MTT assay, Transwell migration and invasion assays, western blotting and a Dual‑Luciferase reporter assay were performed in human OS cell lines. The present study revealed that miR‑150 was downregulated in OS tissues and cell lines. In addition, the expression levels of miR‑150 were correlated with the clinical stage and degree of distant metastasis of patients with OS. In addition, ZEB1 was identified as a direct target of miR‑150 in vitro. In conclusion, miR‑150 targeted ZEB1 to function as an antioncogenic regulator in OS. These findings elucidated a novel underlying mechanism for the pathogenic process in OS carcinogenesis and progression, and may provide novel targeted therapeutic regimens for patients with OS.
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August-2017
Volume 14 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Xu J, Wang Z, Liao Z, Dai D and Ma X: MicroRNA-150 functions as an antioncogenic regulator in osteosarcoma. Oncol Lett 14: 2483-2490, 2017.
APA
Xu, J., Wang, Z., Liao, Z., Dai, D., & Ma, X. (2017). MicroRNA-150 functions as an antioncogenic regulator in osteosarcoma. Oncology Letters, 14, 2483-2490. https://doi.org/10.3892/ol.2017.6393
MLA
Xu, J., Wang, Z., Liao, Z., Dai, D., Ma, X."MicroRNA-150 functions as an antioncogenic regulator in osteosarcoma". Oncology Letters 14.2 (2017): 2483-2490.
Chicago
Xu, J., Wang, Z., Liao, Z., Dai, D., Ma, X."MicroRNA-150 functions as an antioncogenic regulator in osteosarcoma". Oncology Letters 14, no. 2 (2017): 2483-2490. https://doi.org/10.3892/ol.2017.6393