Long non-coding RNA Fer-1-like family member 4 is overexpressed in human glioblastoma and regulates the tumorigenicity of glioma cells

Ding,Feng; Tang,Hongtu; Nie,Dekang; Xia,Liang

doi:10.3892/ol.2017.6403

Long non-coding RNA Fer-1-like family member 4 is overexpressed in human glioblastoma and regulates the tumorigenicity of glioma cells

Authors:
- Feng Ding
- Hongtu Tang
- Dekang Nie
- Liang Xia
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Affiliations: Department of General Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250000, P.R. China, Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, College of Acupuncture and Moxibustion, Hubei University of Chinese Medicine, Wuhan, Hubei 430000, P.R. China, Department of Neurosurgery, Yancheng First Peoples' Hospital, Yancheng, Jiangsu 224001, P.R. China, Department of Neurosurgery, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China
Published online on: June 16, 2017 https://doi.org/10.3892/ol.2017.6403
Pages: 2379-2384

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Abstract

Long non-coding RNA (lncRNA) is a class of regulative non‑coding RNA that is >200 nucleotides in length. Previous studies have demonstrated that lncRNA Fer‑1‑like family member 4 (FER1L4) serves regulatory roles in tumor progression; however, its clinical significance in human neuroglioma remains unclear. In the present study, data from The Cancer Genome Atlas was mined in order to investigate the association between FER1L4 expression and prognosis in patients with glioma. A short interfering (si)RNA targeting FER1L4 was transfected into U373‑MG and U251 glioma cell lines, and cell viability, invasion and apoptosis were examined using CCK‑8, Transwell and Annexin V‑fluorescein isothiocyanate/propidium iodide assays, respectively. FER1L4 was significantly upregulated in high‑grade glioma compared with low‑grade glioma. Additionally, high expression of FER1L4 significantly predicted poor prognosis in patients with glioma. The expression of FER1L4 in glioma cell lines was significantly higher compared with that in normal astrocytes. Furthermore, by downregulating FER1L4 using siRNA, the invasiveness and viability of the glioma cells significantly decreased, while apoptosis significantly increased. The findings from the present study indicate that FER1L4 serves a role in the occurrence and progression of glioma, and could be used as a prognostic biomarker for this disease.

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