MicroRNA‑433 regulates apoptosis by targeting PDCD4 in human osteosarcoma cells

Sun,Yinghua; Wang,Fuchao; Wang,Li; Jiao,Zhaode; Fang,Jun; Li,Jianmin

doi:10.3892/ol.2017.6441

MicroRNA‑433 regulates apoptosis by targeting PDCD4 in human osteosarcoma cells

Authors:
- Yinghua Sun
- Fuchao Wang
- Li Wang
- Zhaode Jiao
- Jun Fang
- Jianmin Li
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Affiliations: Department of Orthopedics, Yidu Central Hospital, Weifang Medical University, Weifang, Shandong 262500, P.R. China, Department of Orthopedics, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China
Published online on: June 21, 2017 https://doi.org/10.3892/ol.2017.6441
Pages: 2353-2358

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Abstract

Osteosarcoma is the most common aggressive sarcoma of the bone in children and adolescents. It is characterized by a high level of genetic instability and recurrent DNA deletions and amplifications. microRNAs (miRNAs) play a key role in cancer initiation, progression and metastasis; however, the potential role of miRNAs in osteosarcoma remains largely unknown. In the present study, miR‑433 was shown to be overexpressed in osteosarcoma tissues compared with normal human osteoblasts. Transfection of miR‑433 mimics into osteosarcoma cell lines significantly decreased apoptosis by targeting programmed cell death 4, a tumor suppressor that is involved in apoptosis. In contrast, inhibition of miR‑433 enhanced apoptosis. Furthermore, in vivo miR‑433 overexpression inhibited the apoptosis of tumor cells and increased tumor growth. The results of the present study suggested that miR‑433 is a potential molecular target for osteosarcoma therapy.

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