Overexpression of long noncoding RNA PEG10 promotes proliferation, invasion and metastasis of hypopharyngeal squamous cell carcinoma

Zhao,Miaoqing; Sun,Dianshui; Li,Xinwei; Xu,Ying; Zhang,Hao; Qin,Yejun; Xia,Ming

doi:10.3892/ol.2017.6498

Overexpression of long noncoding RNA PEG10 promotes proliferation, invasion and metastasis of hypopharyngeal squamous cell carcinoma

Authors:
- Miaoqing Zhao
- Dianshui Sun
- Xinwei Li
- Ying Xu
- Hao Zhang
- Yejun Qin
- Ming Xia
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Affiliations: Department of Pathology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Cancer Center, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China, Institute of Environmental and Occupational Health, Jinan Center for Disease Control and Prevention, Jinan, Shandong 250021, P.R. China, Department of Otorhinolaryngology and Head and Neck Surgery, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China
Published online on: June 30, 2017 https://doi.org/10.3892/ol.2017.6498
Pages: 2919-2925
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the impact of overexpression of long noncoding RNA PEG10 (lncRNA PEG10) on the proliferation, invasion and metastasis of hypopharyngeal squamous cell carcinoma (HSCC). Quantitative reverse transcription polymerase chain reaction was used to quantify lncRNA PEG10 expression levels in HSCC tumor tissues samples, para‑carcinoma tissue samples and the HSCC FaDu cell line. Cell proliferation assays, Transwell invasion assays and wound healing assays were used to evaluate the effects of lncRNA PEG10 on FaDu cells in vitro. In 56 eligible patients, lncRNA PEG10 was expressed at higher levels in HSCC tumor tissues compared with para‑carcinoma tissues, and significant associations were observed between increased tumor expression of lncRNA PEG10 and primary tumor size, lymph node status and tumor node metastasis stage. In the in vitro experimental studies, enhanced expression of lncRNA PEG10 was significantly associated with increased proliferation, invasion and metastasis of FaDu cells. lncRNA PEG10 was upregulated in HSCC, and its overexpression in HSCC cells promoted an increase in the tumorigenic activities of proliferation, invasion and migration. The potential underlying mechanisms require investigation in future studies.

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