Mechanisms of inhibiting human leukemia cell lines by serum of rats treated with compound banmao capsule

Li,Li; Zhu,Lixia; Zhu,Jingjing; Fan,Xiaofen; Ye,Xiujin

doi:10.3892/ol.2017.6688

Mechanisms of inhibiting human leukemia cell lines by serum of rats treated with compound banmao capsule

Authors:
- Li Li
- Lixia Zhu
- Jingjing Zhu
- Xiaofen Fan
- Xiujin Ye
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Affiliations: Department of Hematology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China, Department of Traditional Chinese Medicine, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
Published online on: July 28, 2017 https://doi.org/10.3892/ol.2017.6688
Pages: 4092-4098
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Compound banmao capsule (CBC) is a traditional Chinese medicinal formula composed of extracts from 11 organisms. The present study investigated the mechanism of CBC on the biological behavior of human leukemia cell lines using seropharmacological methods. CBC‑containing rat serum was prepared by intragastrical administration of CBC to rats. The proliferation of human leukemia HL60 and K562 cell lines was assayed by measuring cell viability with the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium method, while cell cycle distribution and the rate of apoptosis were evaluated with flow cytometry. The mRNA expression of vascular endothelial growth factor A (VEGF‑A) and chemotactic and inflammatory genes in human leukemia cell lines was examined using reverse transcription quantitative‑polymerase chain reaction methods. It was revealed that the proliferation of K562 and HL60 cells was significantly inhibited by the CBC‑containing rat serum at 72 h. The CBC‑containing serum also promoted the apoptosis of K562 and HL60 cell lines. The CBC‑containing serum altered the cell cycle progression of K562 and HL60, increasing the proportion of the cells in G1 phase and decreasing the proportion of the cells in S phase. Attenuated expression of VEGF‑A and a decreasing trend in the expression of chemotactic and inflammatory genes were identified following treatment with CBC‑containing serum in HL60 and K562 cells. In conclusion, CBC‑containing serum exerted an inhibitory effect on the growth of K562 and HL60 cells by decreasing cellular proliferation, promoting apoptosis and cell cycle arrest, and decreasing the expression of VEGF‑A, and chemotactic and inflammatory genes.

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1	Vardiman JW: The World Health Organization (WHO) classification of tumors of the hematopoietic and lymphoid tissues: An overview with emphasis on the myeloid neoplasms. Chem Biol Interact. 184:16–20. 2010. View Article : Google Scholar : PubMed/NCBI
2	Li FF, Yi S, Wen L, He J, Yang LJ, Zhao J, Zhang BP, Cui GH and Chen Y: Oridonin induces NPM mutant protein translocation and apoptosis in NPM1c+ acute myeloid leukemia cells in vitro. Acta Pharmacol Sin. 35:806–813. 2014. View Article : Google Scholar : PubMed/NCBI
3	Hu XM, Yuan B, Tanaka S, Song MM, Onda K, Tohyama K, Zhou AX, Toyoda H and Hirano T: Arsenic disulfide-triggered apoptosis and erythroid differentiation in myelodysplastic syndrome and acute myeloid leukemia cell lines. Hematology. 19:352–360. 2014. View Article : Google Scholar : PubMed/NCBI
4	Man S, Gao W, Wei C and Liu C: Anticancer drugs from traditional toxic Chinese medicines. Phytother Res. 26:1449–1465. 2012.PubMed/NCBI
5	Liu D and Chen Z: The effects of cantharidin and cantharidin derivates on tumour cells. Anticancer Agents Med Chem. 9:392–396. 2009. View Article : Google Scholar : PubMed/NCBI
6	Xu H, Wu Q, Peng C and Zhou L: Study on the antiviral activity of San Huang Yi Gan Capsule against hepatitis B virus with seropharmacological method. BMC Complement Altern Med. 13:2392013. View Article : Google Scholar : PubMed/NCBI
7	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
8	Bochu W, Liancai Z and Qi C: Primary study on the application of Serum Pharmacology in Chinese traditional medicine. Colloids Surf B Biointerfaces. 43:194–197. 2005. View Article : Google Scholar : PubMed/NCBI
9	Wu Q, Rong X, Huang P, Han J and Xu H: Study on inhibitory actions of san huang yi gan capsule (SHYGC) on HBeAg with seropharmacological method. Zhong Yao Cai. 23(2): 75–278. 2000.(In Chinese).
10	Zhang YH, Liu JT, Wen BY and Liu N: Mechanisms of inhibiting proliferation of vascular smooth muscle cells by serum of rats treated with Dahuang Zhechong pill. J Ethnopharmacol. 124:125–129. 2009. View Article : Google Scholar : PubMed/NCBI
11	Han SB, Yoon YD, Ahn HJ, Lee HS, Lee CW, Yoon WK, Park SK and Kim HM: Toll-like receptor-mediated activation of B cells and macrophages by polysaccharide isolated from cell culture of Acanthopanax senticosus. Int Immunopharmacol. 3:1301–1312. 2003. View Article : Google Scholar : PubMed/NCBI
12	Wang GS: Medical uses of mylabris in ancient China and recent studies. J Ethnopharmacol. 26:147–162. 1989. View Article : Google Scholar : PubMed/NCBI
13	Tagwireyi D, Ball DE, Loga PJ and Moyo S: Cantharidin poisoning due to ‘Blister beetle’ ingestion. Toxicon. 38:1865–1869. 2000. View Article : Google Scholar : PubMed/NCBI
14	Graziano MJ, Pessah IN, Matsuzawa M and Casida JE: Partial characterization of specific cantharidin binding sites in mouse tissues. Mol Pharmacol. 33:706–712. 1988.PubMed/NCBI
15	Peng F, Wei YQ, Tian L, Yang L, Zhao X, Lu Y, Mao YQ, Kan B, Lei S, Wang GS, et al: Induction of apoptosis by norcantharidin in human colorectal carcinoma cell lines: Involvement of the CD95 receptor/ligand. J Cancer Res Clin Oncol. 128:223–230. 2002. View Article : Google Scholar : PubMed/NCBI
16	Huang Y, Liu Q, Liu K, Yagasaki K and Zhang G: Suppression of growth of highly-metastatic human breast cancer cells by norcantharidin and its mechanisms of action. Cytotechnology. 59:2092009. View Article : Google Scholar : PubMed/NCBI
17	Fan YZ, Fu JY, Zhao ZM and Chen CQ: Inhibitory effect of norcantharidin on the growth of human gallbladder carcinoma GBC-SD cells in vitro. Hepatobiliary Pancreat Dis Int. 6:72–80. 2007.PubMed/NCBI
18	Huan SK, Lee HH, Liu DZ, Wu CC and Wang CC: Cantharidin-induced cytotoxicity and cyclooxygenase 2 expression in human bladder carcinoma cell line. Toxicology. 223:136–143. 2006. View Article : Google Scholar : PubMed/NCBI
19	Dorn DC, Kou CA, Png KJ and Moore MA: The effect of cantharidins on leukemic stem cells. Int J Cancer. 124:2186–2199. 2009. View Article : Google Scholar : PubMed/NCBI
20	Yi SN, Wass J, Vincent P and Iland H: Inhibitory effect of norcantharidin on K562 human myeloid leukemia cells in vitro. Leuk Res. 15:883–886. 1991. View Article : Google Scholar : PubMed/NCBI
21	Schmeda-Hirschmann G, Villaseñor-García MM, Lozoya X and Puebla-Pérez AM: Immunomodulatory activity of Chilean Cyttaria species in mice with L5178Y lymphoma. J Ethnopharmacol. 77:253–257. 2001. View Article : Google Scholar : PubMed/NCBI
22	Schmolz MW, Sacher F and Aicher B: The synthesis of Rantes, G-CSF, IL-4, IL-5, IL-6, IL-12 and IL-13 in human whole-blood cultures is modulated by an extract from Eleutherococcus senticosus L. roots. Phytother Res. 15:268–270. 2001. View Article : Google Scholar : PubMed/NCBI
23	Jeong HJ, Koo HN, Myung NI, Shin MK, Kim JW, Kim DK, Kim KS, Kim HM and Lee YM: Inhibitory effects of mast cell-mediated allergic reactions by cell cultured Siberian Ginseng. Immunopharmacol Immunotoxicol. 23:107–117. 2001. View Article : Google Scholar : PubMed/NCBI
24	Yi JM, Kim MS, Seo SW, Lee KN, Yook CS and Kim HM: Acanthopanax senticosus root inhibits mast cell-dependent anaphylaxis. Clin Chim Acta. 312:163–168. 2001. View Article : Google Scholar : PubMed/NCBI
25	Yoon TJ, Yoo YC, Lee SW, Shin KS, Choi WH, Hwang SH, Ha ES, Jo SK, Kim SH and Park WM: Anti-metastatic activity of Acanthopanax senticosus extract and its possible immunological mechanism of action. J Ethnopharmacol. 93:247–253. 2004. View Article : Google Scholar : PubMed/NCBI
26	Wang Q, Wang Y, Liu R, Yan X, Li Y, Fu H, Bi K and Li Q: Comparison of the effects of Mylabris and Acanthopanax senticosus on promising cancer marker polyamines in plasma of a Hepatoma-22 mouse model using HPLC-ESI-MS. Biomed Chromatogr. 27:208–215. 2013. View Article : Google Scholar : PubMed/NCBI
27	Chang C, Zhu YQ, Mei JJ, Liu SQ and Luo J: Involvement of mitochondrial pathway in NCTD-induced cytotoxicity in human hepG2 cells. J Exp Clin Cancer Res. 29:1452010. View Article : Google Scholar : PubMed/NCBI
28	Zheng LH, Bao YL, Wu Y, Yu CL, Meng X and Li YX: Cantharidin reverses multidrug resistance of human hepatoma HepG2/ADM cells via down-regulation of P-glycoprotein expression. Cancer Lett. 272:102–109. 2008. View Article : Google Scholar : PubMed/NCBI
29	Avramis IA, Kwock R and Avramis VI: Taxotere and vincristine inhibit the secretion of the angiogenesis inducing vascular endothelial growth factor (VEGF) by wild-type and drug-resistant human leukemia T-cell lines. Anticancer Res. 21:2281–2286. 2001.PubMed/NCBI
30	Katoh O, Tauchi H, Kawaishi K, Kimura A and Satow Y: Expression of the vascular endothelial growth factor (VEGF) receptor gene, KDR, in hematopoietic cells and inhibitory effect of VEGF on apoptotic cell death caused by ionizing radiation. Cancer Res. 55:5687–5692. 1995.PubMed/NCBI
31	Ye X, Wang LJ, Lin MF and Ding W: The clinical significance of angiogenesis in the bone marrow of acute leukemia patients. Zhonghua Nei Ke Za Zhi. 42:486–489. 2003.(In Chinese). PubMed/NCBI
32	Ye XJ and Lin MF: Homoharringtonine induces apoptosis of endothelium and down-regulates VEGF expression of K562 cells. J Zhejiang Univ Sci. 5:230–234. 2004. View Article : Google Scholar : PubMed/NCBI
33	Aggarwal BB, Shishodia S, Sandur SK, Pandey MK and Sethi G: Inflammation and cancer: How hot is the link? Biochem Pharmacol. 72:1605–1621. 2006. View Article : Google Scholar : PubMed/NCBI
34	Gasparini C, Celeghini C, Monasta L and Zauli G: NF-κB pathways in hematological malignancies. Cell Mol Life Sci. 71:2083–2102. 2014. View Article : Google Scholar : PubMed/NCBI
35	Candido J and Hagemann T: Cancer-related inflammation. J Clin Immunol. 33:(Suppl 1). S79–S84. 2013. View Article : Google Scholar : PubMed/NCBI