MicroRNA-126 inhibits cell viability and invasion in a diabetic retinopathy model via targeting IRS-1

Fang,Shifeng; Ma,Xiang; Guo,Suping; Lu,Jianmin

doi:10.3892/ol.2017.6695

MicroRNA-126 inhibits cell viability and invasion in a diabetic retinopathy model via targeting IRS-1

Authors:
- Shifeng Fang
- Xiang Ma
- Suping Guo
- Jianmin Lu
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Affiliations: Dalian Medical University, Dalian, Liaoning 116044, P.R. China, Department of Ophthalmology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China
Published online on: August 1, 2017 https://doi.org/10.3892/ol.2017.6695
Pages: 4311-4318

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Abstract

Diabetic retinopathy (DR) is a sight-threatening complication of diabetes. IRS‑1 was predicted to be the target gene of microRNA‑126 (miR‑126). The present study was designed to illustrate the involvement of miR‑126 in the regulation of DR via targeting IRS‑1. The present study revealed that the expression of miR‑126 was significantly decreased while IRS‑1 expression was increased in endothelial cells (ECs) and retinal pericytes (RPs) from a DR mouse model compared with healthy controls. Furthermore, a luciferase reporter assay confirmed the interaction between miR‑126 and IRS‑1. Following transfection with anmiR‑126 mimic or miR‑126 inhibitor, overexpression of miR‑126 was demonstrated to suppress the invasion and viability of ECs and RPs and to inhibit the IRS‑1 and phosphoinositide 3‑kinase (PI3K)/protein kinase B (Akt) pathway protein expression levels, with inhibition of miR‑126 leading to reverse results. Furthermore, transfection with small interfering RNA targeting IRS‑1 altered the miR‑126‑induced effects observed in ECs, indicating that miR‑126 may suppress angiogenesis in DR via inhibition ofIRS‑1 expression. Taken together, the results of the present study suggested that miR‑126 affected the expression of IRS‑1, resulting in downregulated expression of PI3K/Akt pathway proteins, and also suppressed cell invasion and viability. These results may provide a potential therapeutic strategy for DR.

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