Krüppel-like factor 8 induces epithelial-to-mesenchymal transition and promotes invasion of pancreatic cancer cells through transcriptional activation of four and a half LIM-only protein 2

  • Authors:
    • Xiaoping Yi
    • Hongyan Zai
    • Xueying Long
    • Xiaoyi Wang
    • Wenzheng Li
    • Yixiong Li
  • View Affiliations

  • Published online on: August 9, 2017     https://doi.org/10.3892/ol.2017.6734
  • Pages: 4883-4889
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Abstract

Pancreatic cancer (PC) is one of the most aggressive types of cancer with an extremely poor prognosis. Invasive growth and early metastasis is one of the greatest challenges to overcome for the treatment of PC. Numerous previous studies have indicated that the transcription factor Krüppel‑like factor 8 (KLF8) and nuclear cofactor four and a half LIM‑only protein 2 (FHL2) serve important roles in tumorigenesis and tumor progression; however, their roles in PC remain elusive. The present study revealed that KLF8 and FHL2 expression is aberrantly co‑overexpressed in PC tissue samples and associa­ted with tumor metastasis. Furthermore, a positive correlation between the expression levels of KLF8 and FHL2 was observed. Subsequently, the present study identified KLF8 as a critical inducer of epithelial‑to‑mesenchymal transition (EMT) and invasion. Of note, the present study demonstrated that KLF8 overexpression induced a strong increase in FHL2 expression, and subsequent promoter reporter assays determined that KLF8 directly bound and activated the FHL2 gene promoter. Furthermore, FHL2 knockdown in KLF8‑overexpressing cells partially reversed the EMT and invasive phenotypes. The present study identified KLF8-induced FHL2 activation as a novel and critical signaling mechanism underlying human PC invasion.
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October-2017
Volume 14 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yi X, Zai H, Long X, Wang X, Li W and Li Y: Krüppel-like factor 8 induces epithelial-to-mesenchymal transition and promotes invasion of pancreatic cancer cells through transcriptional activation of four and a half LIM-only protein 2. Oncol Lett 14: 4883-4889, 2017
APA
Yi, X., Zai, H., Long, X., Wang, X., Li, W., & Li, Y. (2017). Krüppel-like factor 8 induces epithelial-to-mesenchymal transition and promotes invasion of pancreatic cancer cells through transcriptional activation of four and a half LIM-only protein 2. Oncology Letters, 14, 4883-4889. https://doi.org/10.3892/ol.2017.6734
MLA
Yi, X., Zai, H., Long, X., Wang, X., Li, W., Li, Y."Krüppel-like factor 8 induces epithelial-to-mesenchymal transition and promotes invasion of pancreatic cancer cells through transcriptional activation of four and a half LIM-only protein 2". Oncology Letters 14.4 (2017): 4883-4889.
Chicago
Yi, X., Zai, H., Long, X., Wang, X., Li, W., Li, Y."Krüppel-like factor 8 induces epithelial-to-mesenchymal transition and promotes invasion of pancreatic cancer cells through transcriptional activation of four and a half LIM-only protein 2". Oncology Letters 14, no. 4 (2017): 4883-4889. https://doi.org/10.3892/ol.2017.6734