Downregulation of carbonic anhydrase IV contributes to promotion of cell proliferation and is associated with poor prognosis in non-small cell lung cancer

  • Authors:
    • Jian Chen
    • Lijuan Hu
    • Fan Zhang
    • Junjun Wang
    • Jie Chen
    • Yumin Wang
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  • Published online on: August 10, 2017     https://doi.org/10.3892/ol.2017.6740
  • Pages: 5046-5050
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Abstract

The present study aimed to unveil the biological role of carbonic anhydrase IV (CA IV) and its association with clinical pathological characteristics and prognostic significance in non‑small cell lung cancer (NSCLC). The relative expression level of CA IV was measured by reverse transcription‑quantitative polymerase chain reaction in 114 resected NSCLC tumors and matched adjacent normal tissues and NSCLC cell lines. Overexpression and cell proliferation were assessed in human NSCLC cell lines transfected with CA IV mRNA by lentivirus‑mediated technology. The association of CA IV expression with clinical pathological features and overall survival in 114 cases of NSCLC patients was analyzed. It was demonstrated that CA IV expression was significantly downregulated in NSCLC tumors and six cell lines. Reduced expression of CA IV was significantly correlated with lymph node metastasis. The overall survival of NSCLC patients with low CA IV expression was significantly shorter compared with the high expression group. Overexpression of CA IV suppressed cell proliferation in A549 and NCI‑H1299 cells. The results indicate that low expression of CA IV promotes cell proliferation and serves as an indicator for poor prognosis in NSCLC.
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October-2017
Volume 14 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Chen J, Hu L, Zhang F, Wang J, Chen J and Wang Y: Downregulation of carbonic anhydrase IV contributes to promotion of cell proliferation and is associated with poor prognosis in non-small cell lung cancer. Oncol Lett 14: 5046-5050, 2017
APA
Chen, J., Hu, L., Zhang, F., Wang, J., Chen, J., & Wang, Y. (2017). Downregulation of carbonic anhydrase IV contributes to promotion of cell proliferation and is associated with poor prognosis in non-small cell lung cancer. Oncology Letters, 14, 5046-5050. https://doi.org/10.3892/ol.2017.6740
MLA
Chen, J., Hu, L., Zhang, F., Wang, J., Chen, J., Wang, Y."Downregulation of carbonic anhydrase IV contributes to promotion of cell proliferation and is associated with poor prognosis in non-small cell lung cancer". Oncology Letters 14.4 (2017): 5046-5050.
Chicago
Chen, J., Hu, L., Zhang, F., Wang, J., Chen, J., Wang, Y."Downregulation of carbonic anhydrase IV contributes to promotion of cell proliferation and is associated with poor prognosis in non-small cell lung cancer". Oncology Letters 14, no. 4 (2017): 5046-5050. https://doi.org/10.3892/ol.2017.6740