Clinical significance of ALDH1 combined with DAPI expression in patients with esophageal carcinoma

Chen,Hong; Zhang,Zhi‑Qiang; Zhao,Bin; Ning,Wen‑Long; Wang,Xue‑Yan; Zhou,Fei

doi:10.3892/ol.2017.6743

Clinical significance of ALDH1 combined with DAPI expression in patients with esophageal carcinoma

Authors:
- Hong Chen
- Zhi‑Qiang Zhang
- Bin Zhao
- Wen‑Long Ning
- Xue‑Yan Wang
- Fei Zhou
View Affiliations

Affiliations: Center of Individualized Medicine Diagnosis and Treatment, The First Hospital of Qiqihar, Qiqihar, Heilongjiang 161005, P.R. China, Department of First General Surgery, The First Hospital of Harbin, Harbin, Heilongjiang 150010, P.R. China, Department of Anorectal Surgery, The First Hospital Affiliated of Jiamusi University, Jiamusi, Heilongjiang 154000, P.R. China, Department of Emergency, The First Hospital of Qiqihar, Qiqihar, Heilongjiang 161005, P.R. China, Department of Public Health, The First Hospital of Qiqihar, Qiqihar, Heilongjiang 161005, P.R. China, Department of Second General Surgery, The First Hospital of Qiqihar, Qiqihar, Heilongjiang 161005, P.R. China
Published online on: August 10, 2017 https://doi.org/10.3892/ol.2017.6743
Pages: 4878-4882

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Esophageal carcinoma is the most common type of tumor, with the incidence in China accounting for 50% of cases worldwide and the majority of patients not surviving due to tumor recurrence. According to the cancer stem cell theory, tumor development and recurrence is due to the excitation of a cancer stem cell. Aldehyde dehydrogenase 1 (ALDH1) is an appropriate marker for cancer stem cells and the present study aimed at determining the function of ALDH1 in human esophageal carcinoma. Indirect fluorescence antibody staining was used to investigate the association between the level of ALDH1 protein expression and clinicopathological parameters, including sex, age, vein invasion, degree of tumor cell differentiation and clinical stage. DAPI was used to stain the nuclei of tumor cells and exclude non‑tumor cells. The results of the present study revealed that ALDH1 expression was associated with the level of tumor cell differentiation, tumor‑node‑metastasis stage and lymphatic invasion. In addition, increased expression of ALDH1 was identified in esophageal carcinoma tissues compared with in healthy esophageal tissues. Therefore, ALDH1 may be used as a parameter for the pathology of esophageal carcinoma.

View Figures

View References

1	Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI
2	Guo M, Zhao YD, Yang HJ and Yan XF: Analysis of clinicopathological characteristics for 5406 cases of esophageal neoplasm. Chin J Cancer Prev Treat. 15:54–56. 2008.
3	Debevec L, Jerič T, Kovač V, Bitenc M and Sok M: Is there any progress in routine management of lung cancer patients? A comparative analysis of an institution in 1996 and 2006. Radiol Oncol. 43:47–53. 2009. View Article : Google Scholar
4	Kovac V, Zwitter M and Zagar T: Improved survival after introduction of chemotherapy for malignant pleural mesothelioma in Slovenia: Population-based survey of 444 patients. Radiol Oncol. 46:136–144. 2012. View Article : Google Scholar : PubMed/NCBI
5	Shi HY, Zhu SC, Shen WB and Liu ML: Pathological characteristics of esophageal cancer. Oncol Lett. 8:533–538. 2014.PubMed/NCBI
6	Magni M, Shammah S, Schiró R, Mellado W, Dalla-Favera R and Gianni AM: Induction of cyclophosphamide-resistance by aldehyde-dehydrogenase gene transfer. Blood. 87:1097–1103. 1996.PubMed/NCBI
7	Sophos NA and Vasiliou V: Aldehyde dehydrogenase gene superfamily: The 2002 update. Chem Biol Interact. 143–144:5–22. 2003. View Article : Google Scholar
8	Dylla SJ, Beviglia L, Park IK, Chartier C, Raval J, Ngan L, Pickell K, Aguilar J, Lazetic S, Smith-Berdan S, et al: Colorectal cancer stem cells are enriched in xenogeneic tumors following chemotherapy. PLoS One. 3:e24282008. View Article : Google Scholar : PubMed/NCBI
9	Chute JP, Muramoto GG, Whitesides J, Colvin M, Safi R, Chao NJ and McDonnell DP: Inhibition of aldehyde dehydrogenase and retinoid signaling induces the expansion of human hematopoietic stem cells. Proc Natl Acad Sci USA. 103:11707–11712. 2006. View Article : Google Scholar : PubMed/NCBI
10	Armstrong L, Stojkovic M, Dimmick I, Ahmad S, Stojkovic P, Hole N and Lako M: Phenotypic characterization of murine primitive hematopoietic progenitor cells isolated on basis of aldehyde dehydrogenase activity. Stem Cells. 22:1142–1151. 2004. View Article : Google Scholar : PubMed/NCBI
11	Hess DA, Wirthlin L, Craft TP, Herrbrich PE, Hohm SA, Lahey R, Eades WC, Creer MH and Nolta JA: Selection based on CD133 and high aldehyde dehydrogenase activity isolates long-term reconstituting human hematopoietic stem cells. Blood. 107:2162–2169. 2006. View Article : Google Scholar : PubMed/NCBI
12	Nogami T, Shien T, Tanaka T, Nishiyama K, Mizoo T, Iwamto T, Ikeda H, Taira N, Doihara H and Miyoshi S: Expression of ALDH1 in axillary lymph node metastases is a prognostic factor of poor clinical outcome in breast cancer patients with 1–3 lymph node metastases. Breast Cancer. 21:58–65. 2014. View Article : Google Scholar : PubMed/NCBI
13	Patel M, Lu L, Zander DS, Sreerama L, Coco D and Moreb JS: ALDH1A1 and ALDH3A1b expression in lung cancers: Correlation with histologic type and potential precursors. Lung Cancer. 59:340–349. 2008. View Article : Google Scholar : PubMed/NCBI
14	Zhou F, Mu YD, Liang J, Liu ZX, Chen HS and Zhang JF: Expression and prognostic values of tumor stem cell markers ALDH1 and CD133 in colorectal carcinoma. Oncol Lett. 7:507–512. 2014.PubMed/NCBI
15	Grimason AM, Smith HV, Parker JFW, Bukhari Z, Campbell AT and Robertson LJ: Application of DAPI and immunofluorescence for enhanced identification of Cryptosporidium spp oocysts in water samples. Water Res. 28:733–736. 1994. View Article : Google Scholar
16	Leiker M, Suzuki G, Iyer VS, Canty JM Jr and Lee T: Assessment of a nuclear affinity labeling method for tracking implanted mesenchymal stem cells. Cell Transplant. 17:911–922. 2008. View Article : Google Scholar
17	Ocarino NM, Bozzi A, Pereira RD, Breyner NM, Silva VL, Castanheira P, Goes AM and Serakides R: Behavior of mesenchymal stem cells stained with 4′, 6-diamidino-2-phenylindole dihydrochloride (DAPI) in osteogenic and non osteogenic cultures. Biocell. 32:175–183. 2008.PubMed/NCBI
18	Van Vlierberghe RL, Sandel MH, Prins FA, van Iersel LB, van de Velde CJ, Tollenaar RA and Kuppen PJ: Four-color staining combining fluorescence and brightfield microscopy for simultaneous immune cell phenotyping and localization in tumor tissue sections. Microsc Res Tech. 67:15–21. 2005. View Article : Google Scholar : PubMed/NCBI
19	Huang EH, Hynes MJ, Zhang T, Ginestier C, Dontu G, Appelman H, Fields JZ, Wicha MS and Boman BM: Aldehyde dehydrogenase 1 is a marker for normal and malignant human colonic stem cells (SC) and tracks SC overpopulation during colon tumorigenesis. Cancer Res. 69:3382–3389. 2009. View Article : Google Scholar : PubMed/NCBI
20	National Comprehensive Cancer Network. (NCCN) clinical practice guidelines in oncology. Esophageal and esophagogastric junction cancers. Version 2. 2017.
21	Xu Y, Chen Q, Yu X, Zhou X, Zheng X and Mao W: Factors influencing the risk of recurrence in patients with esophageal carcinoma treated with surgery: A single institution analysis consisting of 1002 cases. Oncol Lett. 5:185–190. 2013.PubMed/NCBI
22	Rubenstein JH and Chen JW: Epidemiology of gastroesophageal reflux disease. Gastroenterol Clin North Am. 43:1–14. 2014. View Article : Google Scholar : PubMed/NCBI
23	Li S, Jiang S, Jiang W, Zhou Y, Shen XY, Luo T, Kong LP and Wang HQ: Anticancer effects of crocetin in human esophageal squamous cell carcinoma KYSE-150 cells. Oncol Lett. 9:1254–1260. 2015.PubMed/NCBI
24	Boman BM and Wicha MS: Cancer stem cells: A step toward the cure. J Clin Oncol. 26:2795–2799. 2008. View Article : Google Scholar : PubMed/NCBI