Involvement of non‑B cell‑derived immunoglobulin G in the metastasis and prognosis of salivary adenoid cystic carcinoma

Peng,Jing; Wang,Hai‑Cheng; Liu,Yang; Jiang,Jiu‑Hui; Lv,Wan‑Qi; Yang,Yue; Li,Cui‑Ying; Qiu,Xiao‑Yan

doi:10.3892/ol.2017.6782

Involvement of non‑B cell‑derived immunoglobulin G in the metastasis and prognosis of salivary adenoid cystic carcinoma

Authors:
- Jing Peng
- Hai‑Cheng Wang
- Yang Liu
- Jiu‑Hui Jiang
- Wan‑Qi Lv
- Yue Yang
- Cui‑Ying Li
- Xiao‑Yan Qiu
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Affiliations: Central Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081, P.R. China, Department of Oral Pathology, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, P.R. China, Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University, Beijing 100191, P.R. China, Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, P.R. China, Department of Dentistry, Capital Medical University Xuanwu Hospital, Beijing 100053, P.R. China
Published online on: August 21, 2017 https://doi.org/10.3892/ol.2017.6782
Pages: 4491-4498
Copyright: © Peng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cancer cell‑derived immunoglobulin G (cancer‑IgG) has been implicated in the pathogenesis and progression of various types of cancer. However, its role in salivary adenoid cystic carcinoma (SACC) remains unclear. The present study aimed to investigate the effects of cancer‑IgG on metastasis and prognosis in 96 patients with SACC. Immunohistochemical staining showed that cancer‑IgG expression was present in all 96 individual SACC tissues. Additionally, high cancer‑IgG expression was significantly correlated with metastasis, nerve invasion and recurrence in SACC (P<0.05). Moreover, cancer‑IgG expression was significantly correlated with the survival duration of patients with SACC (P<0.05). Proliferation, cell motility and invasion all decreased significantly following knockdown of cancer‑IgG in SACC cells (P<0.05) through population‑doubling time, wound healing and transwell invasion assays. Additionally, cancer‑IgG‑knockdown in SACC cells induced the increased expression of E‑cadherin and matrix metalloproteinase 9, and promoted the epithelial‑mesenchymal transition, but decreased the expression of F‑actin filaments. Taken together, these results showed that the high expression of cancer‑IgG was strongly associated with metastasis, recurrence and invasion in SACC, suggesting that cancer‑IgG expression could serve as a useful biomarker to predict the prognosis of the disease.

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1	van der Wal JE, Becking AG, Snow GB and van der Waal I: Distant metastases of adenoid cystic carcinoma of the salivary glands and the value of diagnostic examinations during follow-up. Head Neck. 24:779–783. 2002. View Article : Google Scholar : PubMed/NCBI
2	Tian Z, Li L, Wang L, Hu Y and Li J: Salivary gland neoplasms in oral and maxillofacial regions: A 23-year retrospective study of 6982 cases in an eastern Chinese population. Int J Oral Maxillofac Surg. 39:235–242. 2010. View Article : Google Scholar : PubMed/NCBI
3	Wang YY, Chen WL, Huang ZQ, Yang ZH, Zhang B, Wang JG, Li HG and Li JS: Expression of the membrane-cytoskeletal linker Ezrin in salivary gland adenoid cystic carcinoma. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 112:96–1042. 2011. View Article : Google Scholar : PubMed/NCBI
4	Kokemueller H, Eckardt A, Brachvogel P and Hausamen JE: Adenoid cystic carcinoma of the head and neck - a 20 years experience. Int J Oral Maxillofac Surg. 33:25–31. 2004. View Article : Google Scholar : PubMed/NCBI
5	Liang LZ, Ma B, Liang YJ, Liu HC, Zheng GS, Zhang TH, Chu M, Xu PP, Su YX and Liao GQ: High expression of the autophagy gene Beclin-1 is associated with favorable prognosis for salivary gland adenoid cystic carcinoma. J Oral Pathol Med. 41:621–629. 2012. View Article : Google Scholar : PubMed/NCBI
6	Wang HC, Yang Y, Xu SY, Peng J, Jiang JH and Li CY: The CRISPR/Cas system inhibited the pro-oncogenic effects of alternatively spliced fibronectin extra domain A via editing the genome in salivary adenoid cystic carcinoma cells. Oral Dis. 21:608–618. 2015. View Article : Google Scholar : PubMed/NCBI
7	Ge MH, Ling ZQ, Tan Z, Chen C, Xu JJ and Yu JL: Expression of epidermal growth factor receptor in salivary adenoid cystic carcinoma and its role in cancer invasion. Zhonghua Zhong Liu Za Zhi. 34:278–280. 2012.(In Chinese). PubMed/NCBI
8	Dong L, Wang YX, Li SL, Yu GY, Gan YH, Li D and Wang CY: TGF-beta1 promotes migration and invasion of salivary adenoid cystic carcinoma. J Dent Res. 90:804–809. 2011. View Article : Google Scholar : PubMed/NCBI
9	Arnold A, Cossman J, Bakhshi A, Jaffe ES, Waldmann TA and Korsmeyer SJ: Immunoglobulin-gene rearrangements as unique clonal markers in human lymphoid neoplasms. New Engl J Med. 309:1593–1599. 1983. View Article : Google Scholar : PubMed/NCBI
10	Qiu X, Zhu X, Zhang L, Mao Y, Zhang J, Hao P, Li G, Lv P, Li Z, Sun X, et al: Human epithelial cancers secrete immunoglobulin g with unidentified specificity to promote growth and survival of tumor cells. Cancer Res. 63:6488–6495. 2003.PubMed/NCBI
11	Zheng S, Cao J and Geng L: Structure and expression of colorectal cancer related Immunoglobulin novel gene SNC73. Zhonghua Yi Xue Za Zhi. 81:485–488. 2001.(In Chinese). PubMed/NCBI
12	Chen Z and Gu J: Immunoglobulin G expression in carcinomas and cancer cell lines. FASEB J. 21:2931–2938. 2007. View Article : Google Scholar : PubMed/NCBI
13	Jiang C, Huang T, Wang Y, Huang G, Wan X and Gu J: Immunoglobulin G expression in lung cancer and its effects on metastasis. PLoS One. 9:e973592014. View Article : Google Scholar : PubMed/NCBI
14	Liu Y, Chen Z, Niu N, Chang Q, Deng R, Korteweg C and Gu J: IgG gene expression and its possible significance in prostate cancers. Prostate. 72:690–701. 2012. View Article : Google Scholar : PubMed/NCBI
15	Kimoto Y: Expression of heavy-chain constant region of immunoglobulin and T-cell receptor gene transcripts in human non-hematopoietic tumor cell lines. Genes Chromosomes Cancer. 22:83–86. 1998. View Article : Google Scholar : PubMed/NCBI
16	Zhu X, Li C, Sun X, Mao Y, Li G, Liu X, Zhang Y and Qiu X: Immunoglobulin mRNA and protein expression in human oral epithelial tumor cells. Appl Immunohistochem Mol Morphol. 16:232–238. 2008. View Article : Google Scholar : PubMed/NCBI
17	Lee G and Ge B: Cancer cell expressions of immunoglobulin heavy chains with unique carbohydrate-associated biomarker. Cancer Biomark. 5:177–188. 2009. View Article : Google Scholar : PubMed/NCBI
18	Li SL: Establishment of a human cancer cell line from adenoid cystic carcinoma of the minor salivary gland. Zhonghua Kou Qiang Yi Xue Za Zhi. 25:29–31. 1990.(In Chinese). PubMed/NCBI
19	Babbage G, Ottensmeier CH, Blaydes J, Stevenson FK and Sahota SS: Immunoglobulin heavy chain locus events and expression of activation-induced cytidine deaminase in epithelial breast cancer cell lines. Cancer Res. 66:3996–4000. 2006. View Article : Google Scholar : PubMed/NCBI
20	Liu Y, Liu D, Wang C, Liao Q, Huang J, Jiang D, Shao W, Yin CC, Zhang Y, Lee G and Qiu X: Binding of the monoclonal antibody RP215 to immunoglobulin G in metastatic lung adenocarcinomas is correlated with poor prognosis. Histopathology. 67:645–653. 2015. View Article : Google Scholar : PubMed/NCBI
21	Liao Q, Liu W, Liu Y, Wang F, Wang C, Zhang J, Chu M, Jiang D, Xiao L, Shao W, et al: Aberrant high expression of immunoglobulin G in epithelial stem/progenitor-like cells contributes to tumor initiation and metastasis. Oncotarget. 6:40081–40094. 2015. View Article : Google Scholar : PubMed/NCBI