Hispolon as an inhibitor of TGF‑β‑induced epithelial‑mesenchymal transition in human epithelial cancer cells by co‑regulation of TGF‑β‑Snail/Twist axis

Hong,Darong; Park,Min‑Ju; Jang,Eun  Hyang; Jung,Bom; Kim,Nam‑Jung; Kim,Jong‑Ho

doi:10.3892/ol.2017.6789

Hispolon as an inhibitor of TGF‑β‑induced epithelial‑mesenchymal transition in human epithelial cancer cells by co‑regulation of TGF‑β‑Snail/Twist axis

Authors:
- Darong Hong
- Min‑Ju Park
- Eun Hyang Jang
- Bom Jung
- Nam‑Jung Kim
- Jong‑Ho Kim
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Affiliations: Department of Life and Nanopharmaceutical Science, Graduate School, Kyung Hee University, Dongdaemun‑gu, Seoul 02447, Republic of Korea, Department of Pharmacy, Graduate School, Kyung Hee University, Dongdaemun‑gu, Seoul 02447, Republic of Korea
Published online on: August 23, 2017 https://doi.org/10.3892/ol.2017.6789
Pages: 4866-4872

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Abstract

Hispolon (HPL), isolated from Phellinus linteus, has been used to treat various types of pathology, including inflammation, gastroenteric disorders, lymphatic diseases and numerous cancer subtypes. HPL has previously been reported to demonstrate a significant therapeutic efficacy against various types of cancer cells, including melanoma, leukemia, hepatocarcinoma, bladder and gastric cancer cells. However, its potential role in the epithelial‑mesenchymal transition (EMT) has not been demonstrated. The present study investigated the effects of HPL on the EMT. Transforming growth factor β (TGF‑β) induced enhanced cell migration and invasion, EMT‑associated phenotypic changes. In the present study, HPL recovered the reduction of E‑cadherin expression level in TGF‑β treated cancer cells, which was regulated by the expression of Snail and Twist. HPL downregulated Snail and Twist, an effect that was enhanced by TGF‑β. These findings provide novel evidence that HPL suppresses cancer cell migration and invasion by inhibiting EMT. Therefore, HPL may be a potent anticancer agent, inhibiting metastasis.

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