FHIT promoter DNA methylation and expression analysis in childhood acute lymphoblastic leukemia

  • Authors:
    • Gholamreza Bahari
    • Mohammad Hashemi
    • Majid Naderi
    • Simin Sadeghi‑Bojd
    • Mohsen Taheri
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  • Published online on: August 23, 2017     https://doi.org/10.3892/ol.2017.6796
  • Pages: 5034-5038
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Abstract

Fragile histidine triad (FHIT) is a tumor suppressor gene, which is involved in several malignancies. Epigenetic alterations in FHIT have been hypothesized to contribute to tumorigenesis. The present study aimed to examine DNA promoter methylation and gene expression levels of FHIT in childhood acute lymphoblastic leukemia (ALL), in a sample of Iranian patients. The promoter methylation status of FHIT was analyzed in 100 patients diagnosed with ALL and 120 healthy control patients. mRNA expression levels were assessed in 30 new cases of ALL compared with 32 healthy controls. Hypermethylation of the FHIT promoter was significantly more frequent in patients with ALL than in healthy controls (OR=3.83, 95% CI=1.51‑9.75, P=0.007). Furthermore, FHIT mRNA expression levels were significantly reduced in childhood ALL patients compared with healthy controls (P=0.032). The results of the present study revealed that dysregulation of the FHIT gene may contribute to the pathogenesis of childhood ALL. Future studies investigating a larger sample population with greater ethnic diversity would be beneficial, to confirm the results from the present study.
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October-2017
Volume 14 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Bahari G, Hashemi M, Naderi M, Sadeghi‑Bojd S and Taheri M: FHIT promoter DNA methylation and expression analysis in childhood acute lymphoblastic leukemia. Oncol Lett 14: 5034-5038, 2017.
APA
Bahari, G., Hashemi, M., Naderi, M., Sadeghi‑Bojd, S., & Taheri, M. (2017). FHIT promoter DNA methylation and expression analysis in childhood acute lymphoblastic leukemia. Oncology Letters, 14, 5034-5038. https://doi.org/10.3892/ol.2017.6796
MLA
Bahari, G., Hashemi, M., Naderi, M., Sadeghi‑Bojd, S., Taheri, M."FHIT promoter DNA methylation and expression analysis in childhood acute lymphoblastic leukemia". Oncology Letters 14.4 (2017): 5034-5038.
Chicago
Bahari, G., Hashemi, M., Naderi, M., Sadeghi‑Bojd, S., Taheri, M."FHIT promoter DNA methylation and expression analysis in childhood acute lymphoblastic leukemia". Oncology Letters 14, no. 4 (2017): 5034-5038. https://doi.org/10.3892/ol.2017.6796