The role of LIM and SH3 protein‑1 in bladder cancer metastasis

Sato,Misaki; Yoneyama,Mihoko  Sutoh; Hatakeyama,Shingo; Funyu,Tomihisa; Suzuki,Tadashi; Ohyama,Chikara; Tsuboi,Shigeru

doi:10.3892/ol.2017.6802

The role of LIM and SH3 protein‑1 in bladder cancer metastasis

Authors:
- Misaki Sato
- Mihoko Sutoh Yoneyama
- Shingo Hatakeyama
- Tomihisa Funyu
- Tadashi Suzuki
- Chikara Ohyama
- Shigeru Tsuboi
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Affiliations: Department of Cancer Immunology and Cell Biology, Oyokyo Kidney Research Institute, Hirosaki, Aomori 036‑8243, Japan, Department of Urology, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori 036‑8562, Japan
Published online on: August 23, 2017 https://doi.org/10.3892/ol.2017.6802
Pages: 4829-4834

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Abstract

The LIM and SH3 protein‑1 (LASP‑1) is a multi‑domain protein that is involved in several malignant cancers. The role of LASP‑1 in malignant phenotypes including high invasive properties and unrestricted cell proliferation, remain to be elucidated. The present study reported the association of LASP‑1 expression with bladder cancer malignancy and its role in cancer cell invasion and proliferation. The immunohistochemical analysis of the expression status of LASP‑1 in radical cystectomy specimens from invasive bladder cancer patients revealed that the LASP‑1‑positive patients demonstrated a decreased survival rate compared with the LASP‑1‑negative patients. The expression level of LASP‑1 was increased in invasive bladder cancer cell lines compared with the non‑invasive bladder cancer cell lines. Invasive cancer cells form invadopodia, the filamentous actin‑based membrane protrusions that are essential in cancer cell invasion. Knockdown of LASP‑1 reduced the ability to form invadopodia, resulting in decreased invasive capacity of the LASP‑1 knockdown cells. In addition, knockdown of LASP‑1 reduced cell proliferation. These results suggest that LASP‑1 is important in invadopodia formation and cell proliferation of bladder cancer cells, promoting the malignant properties and resulting in poor‑prognosis.

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