miR‑514a‑3p functions as a tumor suppressor in renal cell carcinoma

  • Authors:
    • Lu Jin
    • Yifan Li
    • Zeng Zhang
    • Tao He
    • Jia Hu
    • Jiaju Liu
    • Mingwei Chen
    • Yaoting Gui
    • Shangqi Yang
    • Xiangming Mao
    • Yun Chen
    • Yongqing Lai
  • View Affiliations

  • Published online on: August 31, 2017     https://doi.org/10.3892/ol.2017.6855
  • Pages: 5624-5630
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Abstract

Renal cell carcinoma (RCC) is the most common type of kidney cancer, and the prognosis of metastatic RCC remains poor with a high rate of recurrence and mortality. A previous study has revealed that microRNA (miRNA), which negatively regulates protein expression, serves a role of oncogene or tumor suppressor. The aim of the present study was to investigate the expression and function of miR‑514a‑3p in RCC. To detect the expression of miR‑514a‑3p in 32 paired RCC tissues, quantitative polymerase chain reaction (qPCR) was performed. The function of miR‑514a‑3p in the proliferation, mobility and apoptosis of RCC cells (786‑O and ACHN) was assessed by MTT, CCK‑8, cell scratch, Transwell, Hoechst 33342 staining and flow cytometry assay. The results of qPCR revealed that miR‑514a‑3p was significantly downregulated in RCC tissues compared with adjacent normal tissues. Upregulation of miR‑514a‑3p by transfection of mimics suppressed RCC cell proliferation, migration and invasion, and induced cell apoptosis. The results revealed that miR‑514a‑3p was significantly downregulated in RCC and may serve a role as tumor suppressor in RCC. Further studies are required, focusing on the possibility of using miR‑514a‑3p as a biomarker for RCC as well as the pathway of miR‑514a‑3p in RCC.
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November-2017
Volume 14 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Jin L, Li Y, Zhang Z, He T, Hu J, Liu J, Chen M, Gui Y, Yang S, Mao X, Mao X, et al: miR‑514a‑3p functions as a tumor suppressor in renal cell carcinoma. Oncol Lett 14: 5624-5630, 2017.
APA
Jin, L., Li, Y., Zhang, Z., He, T., Hu, J., Liu, J. ... Lai, Y. (2017). miR‑514a‑3p functions as a tumor suppressor in renal cell carcinoma. Oncology Letters, 14, 5624-5630. https://doi.org/10.3892/ol.2017.6855
MLA
Jin, L., Li, Y., Zhang, Z., He, T., Hu, J., Liu, J., Chen, M., Gui, Y., Yang, S., Mao, X., Chen, Y., Lai, Y."miR‑514a‑3p functions as a tumor suppressor in renal cell carcinoma". Oncology Letters 14.5 (2017): 5624-5630.
Chicago
Jin, L., Li, Y., Zhang, Z., He, T., Hu, J., Liu, J., Chen, M., Gui, Y., Yang, S., Mao, X., Chen, Y., Lai, Y."miR‑514a‑3p functions as a tumor suppressor in renal cell carcinoma". Oncology Letters 14, no. 5 (2017): 5624-5630. https://doi.org/10.3892/ol.2017.6855