Expression of NF-κB and PTEN in osteosarcoma and its clinical significance

Gong,Teng; Su,Xuetao; Xia,Qun; Wang,Jinggui; Kan,Shilian

doi:10.3892/ol.2017.6972

Expression of NF-κB and PTEN in osteosarcoma and its clinical significance

Authors:
- Teng Gong
- Xuetao Su
- Qun Xia
- Jinggui Wang
- Shilian Kan
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Affiliations: Department of Spinal Surgery, Tianjin Hospital, Tianjin 300211, P.R. China, Department of Orthopaedic Surgery, The Affiliated Hospital of Logistics College of Chinese People's Armed Police Forces, Tianjin 300162, P.R. China
Published online on: September 15, 2017 https://doi.org/10.3892/ol.2017.6972
Pages: 6744-6748
Copyright: © Gong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

We investigated the role of nuclear factor-κB (NF-κB) and phosphatase and tensin homolog deleted in chromosome 10 (PTEN) in the pathogenesis of osteosarcoma and its relationship with prognosis. Immunohistochemical method was used to detect the expression of NF-κB and PTEN in osteosarcoma and adjacent tissues. RT-PCR was used to detect the expression of NF-κB and PTEN mRNA in osteosarcoma and adjacent tissues. Western blotting was used to detect the expression of NF-κB and PTEN in osteosarcoma and adjacent tissues and compare their differences. The expression of NF-κB and PTEN was detected in osteosarcoma and adjacent tissues. The positive rate of NF-κB was 75.3 and 32.9%, respectively; while the positive rate of PTEN was 67.1 and 90.4%, respectively. The positive expression of NF-κB and PTEN was statistically significant. There was a negative correlation between NF-κB and PTEN expression (r=-0.502, p<0.05). The positive and negative expression of NF-κB and PTEN was statistically significant for the five-year survival (p<0.05). At gene and protein level, osteosarcoma tissues had higher expression of NF-κB, and lower expression of PTEN, which was significantly different from the adjacent tissues. In osteosarcoma, NF-κB is highly expressed, but PTEN is expressed at low level, and the two are negatively correlated. This is of great significance for the early diagnosis of osteosarcoma and prognosis.

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