Artesunate inhibits proliferation and invasion of mouse hemangioendothelioma cells in vitro and of tumor growth in vivo

  • Authors:
    • Ning Wang
    • Hongxia Chen
    • Yinping Teng
    • Xionghui Ding
    • Huan Wu
    • Xianqing Jin
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  • Published online on: September 18, 2017     https://doi.org/10.3892/ol.2017.6986
  • Pages: 6170-6176
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Abstract

Artesunate has been demonstrated to be a novel potential antitumor agent in numerous studies. However, its efficacy in infantile hemangioma is unknown. The aim of the present study was to investigate the role of artesunate in the control of vascular tumor biological behavior and molecular mechanism using mouse hemangioendothelioma endothelial (EOMA) cells and a nude mouse model. Cell viability, apoptosis and invasion were determined by an MTT assay, flow cytometric analysis and Transwell invasion assay, respectively. Reverse transcription‑quantitative polymerase chain reaction and western blotting were utilized to examine the expression of genes and proteins. Inoculated EOMA cells were injected into the subcutaneous tissues of nude mice to observe the effect of artesunate therapy on the vascular tumor, an effect that was similar to that of pingyangmycin (PYM). It was identified that artesunate treatment (0‑600 µg/ml) inhibited cell growth in a time‑ and dose‑dependent manner. Artesunate at 300 µg/ml significantly reduced the proliferation and invasion of EOMA cells, and significantly decreased the expression of vascular endothelial growth factor (VEGF)‑A, VEGFR‑1, VEGFR‑2 and hypoxia inducible factor‑1α over time; caspase‑3 was simultaneously upregulated in vitro. Artesunate significantly inhibited tumor growth, and the curative effect was similar to that observed with PYM in vivo. It was concluded that artesunate could effectively inhibit the growth of vascular tumors, and thus could be a novel drug candidate for the treatment of infantile hemangioma.
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November-2017
Volume 14 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wang N, Chen H, Teng Y, Ding X, Wu H and Jin X: Artesunate inhibits proliferation and invasion of mouse hemangioendothelioma cells in vitro and of tumor growth in vivo. Oncol Lett 14: 6170-6176, 2017.
APA
Wang, N., Chen, H., Teng, Y., Ding, X., Wu, H., & Jin, X. (2017). Artesunate inhibits proliferation and invasion of mouse hemangioendothelioma cells in vitro and of tumor growth in vivo. Oncology Letters, 14, 6170-6176. https://doi.org/10.3892/ol.2017.6986
MLA
Wang, N., Chen, H., Teng, Y., Ding, X., Wu, H., Jin, X."Artesunate inhibits proliferation and invasion of mouse hemangioendothelioma cells in vitro and of tumor growth in vivo". Oncology Letters 14.5 (2017): 6170-6176.
Chicago
Wang, N., Chen, H., Teng, Y., Ding, X., Wu, H., Jin, X."Artesunate inhibits proliferation and invasion of mouse hemangioendothelioma cells in vitro and of tumor growth in vivo". Oncology Letters 14, no. 5 (2017): 6170-6176. https://doi.org/10.3892/ol.2017.6986