Inhibition of prostate cancer cell growth in vivo with short hairpin RNA targeting SATB1

Wang,Qiang; Hu,Shi‑Cheng; Yang,Chun‑Sheng; Chen,Jia‑Cun; Zheng,Jun‑Nian; Sun,Xiao‑Qing; Wang,Jun‑Qi

doi:10.3892/ol.2017.7006

Inhibition of prostate cancer cell growth in vivo with short hairpin RNA targeting SATB1

Authors:
- Qiang Wang
- Shi‑Cheng Hu
- Chun‑Sheng Yang
- Jia‑Cun Chen
- Jun‑Nian Zheng
- Xiao‑Qing Sun
- Jun‑Qi Wang
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Affiliations: The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210046, P.R. China, Department of Urology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002, P.R. China, Department of Dermatology, Affiliated Huai'an Hospital of Xuzhou Medical University, The Second People's Hospital of Huai'an, Huai'an 223002, P.R. China, Center of Clinical Oncology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002, P.R. China
Published online on: September 20, 2017 https://doi.org/10.3892/ol.2017.7006
Pages: 6592-6596
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Despite previous advances, the treatment options for prostate cancer remain limited. For the purposes of gene knockdown, the utility of RNA interference has been demonstrated and is considered to have therapeutic potential. In the present study, a short hairpin RNA (shRNA) was used to assess the effect of special AT‑rich sequence binding protein (SATB1) downregulation on the growth and metastatic potential of prostate cancer in xenograft nude mice. A plasmid carrying shRNA targeting SATB1, pSilencer‑SATB1‑shRNA, was successfully engineered. Using this plasmid, significant downregulation of SATB1 mRNA and protein expression in the DU145 prostate cancer cells was observed. pSilencer‑SATB1‑shRNA was demonstrated to be markedly efficacious against prostate cancer xenografts in nude mice. These results may lead to a novel method of improving gene therapy efficacy against prostate cancer via regulating the function of SATB1.

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1	Siegel R, Naishadham D and Jemal A: Cancer statistics, 2013. CA Cancer J Clin. 63:11–30. 2013. View Article : Google Scholar : PubMed/NCBI
2	Shore N: Management of early-stage prostate cancer. Am J Manag Care. 20 12 Suppl:S260–S272. 2014.PubMed/NCBI
3	Gridley DS and Slater JM: Gene therapy: A possible aid to cancer radiotherapy. Discov Med. 4:408–414. 2004.PubMed/NCBI
4	Altaner C: Prodrug cancer gene therapy. Cancer Lett. 270:191–201. 2008. View Article : Google Scholar : PubMed/NCBI
5	Tangney M: Gene therapy for cancer: Dairy bacteria as delivery vectors. Discov Med. 10:195–200. 2010.PubMed/NCBI
6	Singh P, Yam M, Russell PJ and Khatri A: Molecular and traditional chemotherapy: A united front against prostate cancer. Cancer Lett. 293:1–14. 2010. View Article : Google Scholar : PubMed/NCBI
7	Ambesajir A, Kaushik A, Kaushik JJ and Petros ST: RNA interference: A futuristic tool and its therapeutic applications. Saudi J Biol Sci. 19:395–403. 2012. View Article : Google Scholar : PubMed/NCBI
8	Bora RS, Gupta D, Mukkur TK and Saini KS: RNA interference therapeutics for cancer: Challenges and opportunities (review). Mol Med Rep. 6:9–15. 2012.PubMed/NCBI
9	Mansoori B, Shotorbani Sandoghchian S and Baradaran B: RNA interference and its role in cancer therapy. Adv Pharm Bull. 4:313–321. 2014.PubMed/NCBI
10	Agrawal N, Dasaradhi PV, Mohmmed A, Malhotra P, Bhatnagar RK and Mukherjee SK: RNA interference: Biology, mechanism, and applications. Microbiol Mol Biol Rev. 67:657–685. 2003. View Article : Google Scholar : PubMed/NCBI
11	Lee Y, Ahn C, Han J, Choi H, Kim J, Yim J, Lee J, Provost P, Rådmark O, Kim S and Kim VN: The nuclear RNase III Drosha initiates microRNA processing. Nature. 425:415–419. 2003. View Article : Google Scholar : PubMed/NCBI
12	Kohwi-Shigematsu T, Poterlowicz K, Ordinario E, Han HJ, Botchkarev VA and Kohwi Y: Genome organizing function of SATB1 in tumor progression. Semin Cancer Biol. 23:72–79. 2013. View Article : Google Scholar : PubMed/NCBI
13	Yasui D, Miyano M, Cai S, Varga-Weisz P and Kohwi-Shigematsu T: SATB1 targets chromatin remodelling to regulate genes over long distances. Nature. 419:641–645. 2002. View Article : Google Scholar : PubMed/NCBI
14	Han HJ, Russo J, Kohwi Y and Kohwi-Shigematsu T: SATB1 reprogrammes gene expression to promote breast tumour growth and metastasis. Nature. 452:187–193. 2008. View Article : Google Scholar : PubMed/NCBI
15	Mao L, Yang C, Wang J, Li W, Wen R, Chen J and Zheng J: SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion. J Transl Med. 11:1112013. View Article : Google Scholar : PubMed/NCBI
16	Shukla S, Sharma H, Abbas A, MacLennan GT, Fu P, Danielpour D and Gupta S: Upregulation of SATB1 is associated with prostate cancer aggressiveness and disease progression. PLoS One. 8:e535272013. View Article : Google Scholar : PubMed/NCBI
17	Mao LJ, Zhang J, Liu N, Fan L, Yang DR, Xue BX, Shan YX and Zheng JN: Oncolytic virus carrying shRNA targeting SATB1 inhibits prostate cancer growth and metastasis. Tumour Biol. 36:9073–9081. 2015. View Article : Google Scholar : PubMed/NCBI