Aurora kinase A inhibitor TCS7010 demonstrates pro‑apoptotic effect through the unfolded protein response pathway in HCT116 colon cancer cells

Lee,Da  Hyun; Kim,Chang  Gun; Lim,Yoongho; Shin,Soon  Young

doi:10.3892/ol.2017.7023

Aurora kinase A inhibitor TCS7010 demonstrates pro‑apoptotic effect through the unfolded protein response pathway in HCT116 colon cancer cells

Authors:
- Da Hyun Lee
- Chang Gun Kim
- Yoongho Lim
- Soon Young Shin
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Affiliations: Department of Biological Sciences, College of Biological Science and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea, Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul 05029, Republic of Korea, Cancer and Metabolism Institute, Konkuk University, Seoul 05029, Republic of Korea
Published online on: September 22, 2017 https://doi.org/10.3892/ol.2017.7023
Pages: 6571-6577
Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Aurora kinase A (AURKA) is essential for regulating mitosis and is frequently amplified in various cancer cell types. However, the effect of AURKA inhibition on the induction of apoptosis remains unclear. In the present study, it was reported that treatment with TCS7010, a specific inhibitor of AURKA, resulted in the accumulation of cells in the sub‑G0/G1 phase of the cell cycle and increased the percentage of annexin V‑binding cells. The cleavage of caspase‑2, caspase‑7, and poly(ADP‑ribose)polymerase (PARP) significantly increased in a time‑dependent manner following TCS7010 treatment. In addition, TCS7010 resulted in the production of reactive oxygen species (ROS) and stimulation of the unfolded protein response (UPR), leading to the upregulation of CCAAT/enhancer‑binding protein‑homologous protein (CHOP), and its downstream target BCL2 like 11 (BIM). Pretreatment with N‑acetylcystein, a ROS scavenger, significantly abrogated TCS7010‑induced accumulation of CHOP, BIM, cleaved caspase‑7 and cleaved PARP. These results suggest that TCS7010 triggers apoptosis through the ROS‑mediated UPR signaling pathway.

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