Effects of andrographolide on postoperative cognitive dysfunction and the association with NF‑κB/MAPK pathway

Ding,Yongbo; Shi,Cunxian; Chen,Linjing; Ma,Piliang; Li,Kezhong; Jin,Jin; Zhang,Qingfeng; Li,Aizhi

doi:10.3892/ol.2017.7088

Effects of andrographolide on postoperative cognitive dysfunction and the association with NF‑κB/MAPK pathway

Authors:
- Yongbo Ding
- Cunxian Shi
- Linjing Chen
- Piliang Ma
- Kezhong Li
- Jin Jin
- Qingfeng Zhang
- Aizhi Li
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Affiliations: Department of Anesthesiology, School of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China, Department of Operating Room, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China, Department of Anesthesiology, Qingdao Hiser Medical Center, Qingdao, Shandong 266033, P.R. China, Department of Anesthesiology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China
Published online on: September 27, 2017 https://doi.org/10.3892/ol.2017.7088
Pages: 7367-7373
Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study investigated the effects of andrographolide on postoperative cognitive dysfunction (POCD) in aged rats to gain insight of the underlying mechanism, which may provide theoretical basis for the clinical application of andrographolide to prevent POCD in older patients. Thirty aged male rats were randomly assigned to 3 groups: Control, model and andrographolide groups. The Morris water maze test was used to examine the spatial memory and learning ability of the rats postoperatively. The histological alterations of neuronal cells in the hippocampus were visualized by H&E staining. The serum levels of neuron‑specific enolase (NSE), human soluble protein‑100β (S‑100β) and the inflammation factors of interluekin (IL)‑1β, IL‑6 and TNF‑α involved in the nuclear factor κB (NF‑κB)/mitogen‑activated protein kinase (MAPK) signaling pathway were detected by ELISA. The NF‑κB/MAPK signaling pathway‑associated proteins in rat serum were detected by western blotting. Following andrographolide treatment, the rats significantly gained learning ability after surgery. Is it ameliorated hippocampal neuronal injury in rats following surgery. Andrographolide decreased NSE, S‑100β, and the inflammation factors, IL‑6, IL‑1β and TNF‑α in serum. Andrographolide reduced NF‑κB/MAPK pathway‑associated protein expression. Andrographolide ameliorated POCD in aged rats following surgery. The underlying mechanism may be associated with the downregulation the inflammatory factors and NF‑κB/MAPK‑associated protein expression.

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