microRNA‑34a overexpression inhibits cell migration and invasion via regulating SIRT1 in hepatocellular carcinoma Corrigendum in /10.3892/ol.2019.11048

Zhou,Jianhui; Zhou,Wenying; Kong,Fangen; Xiao,Xiaoyu; Kuang,Haoyu; Zhu,Yingxian

doi:10.3892/ol.2017.7090

microRNA‑34a overexpression inhibits cell migration and invasion via regulating SIRT1 in hepatocellular carcinoma

Corrigendum in: /10.3892/ol.2019.11048

Authors:
- Jianhui Zhou
- Wenying Zhou
- Fangen Kong
- Xiaoyu Xiao
- Haoyu Kuang
- Yingxian Zhu
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Affiliations: Department of Clinical Laboratory, Fifth Affiliated Hospital of Sun Yat‑Sen University, Zhuhai, Guangdong 519000, P.R. China, Department of Central Laboratory, Fifth Affiliated Hospital of Sun Yat‑Sen University, Zhuhai, Guangdong 519000, P.R. China, Department of Neurosurgery, Fifth Affiliated Hospital of Sun Yat‑Sen University, Zhuhai, Guangdong 519000, P.R. China, Department of Anesthesiology, Fifth Affiliated Hospital of Sun Yat‑Sen University, Zhuhai, Guangdong 519000, P.R. China
Published online on: September 28, 2017 https://doi.org/10.3892/ol.2017.7090
Pages: 6950-6954

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Abstract

Hepatocellular carcinoma (HCC) remains one of the most common types of malignancy with high mortality and morbidity rates. Previous studies have suggested that microRNAs (miRs) serve pivotal functions in various types of tumor. The aim of the present study was to assess the association between miR‑34a expression and HCC cell migration and invasion, and the potential underlying mechanisms. The miR‑34a overexpression vector or scramble control was transfected into human Hep3B and Huh7 cell lines. Transwell assays, and Matrigel and wound healing assays were used to detect the effects of miR‑34a expression on HCC cell invasion and migration, respectively. The expression of miR‑34a and the mRNA expression of other associated proteins were detected using quantitative reverse transcription polymerase chain reaction, and protein levels were measured using western blot analysis. Compared with the control, miR‑34a expression was significantly downregulated in Hep3B and Huh7 cells, but this was reversed by the transfection with exogenous miR‑34a (P<0.01). The number of migrated or invaded cells was significantly reduced by the overexpression of miR‑34a in Hep3B or Huh7 cells (P<0.01). The expression of sirtuin 1 was upregulated, while the level of acetylate‑p53 was downregulated by overexpression of miR‑34a. Taken together, the results of the present study suggested that the overexpression of miR‑34a may have suppressed HCC metastasis via inhibited cell migration and invasion.

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