Study of miR-10b regulatory mechanism for epithelial-mesenchymal transition, invasion and migration in nasopharyngeal carcinoma cells

Wang,Weiyi

doi:10.3892/ol.2017.7172

Study of miR-10b regulatory mechanism for epithelial-mesenchymal transition, invasion and migration in nasopharyngeal carcinoma cells

Authors:
- Weiyi Wang
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Affiliations: Department of Ear-Nose-Throat, Hunan Provincial People's Hospital, Changsha, Hunan 410005, P.R. China
Published online on: October 11, 2017 https://doi.org/10.3892/ol.2017.7172
Pages: 7207-7210
Copyright: © Wang . This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the study was to investigate the miR-10b regulatory mechanism for epithelial-mesenchymal transition (EMT) and its effect on the proliferation and migration of nasopharyngeal carcinoma cells. RT-qPCR was used to detect the expression of miR-10b in CNE1 nasopharyngeal carcinoma cell line. The NP69 nasopharyngeal mucosal cell line was used to determine the expression of miR-10b after infection with lentivirus. The effect of miR-10b on the proliferation of NP69 was examined using cell counting kit-8. The effect of miR-10b on NP69 migration was examined using scratch assay. Western blot analysis was used to detect the effects of miR-10b on the expression of epithelial cell markers E-cadherin and β-catenin and mesenchymal cell markers fibronectin, N-cadherin, vimentin and matrix metalloproteinase-9 (MMP-9). The present study showed that miR-10b was highly expressed in CNE1 cells. The stable expression of miR-10b promoted the proliferation and migration of NP69 cells, downregulated the expression of epithelial cell markers E-cadherin and β-catenin, and upregulated the expression of mesenchymal cell markers fibronectin, N-cadherin, vimentin and MMP-9 resulting in cell EMT. In conclusion, miR-10b promotes the proliferation and migration of nasopharyngeal carcinoma cells, and induces EMT in nasopharyngeal carcinoma cells, thereby having the potential to become a new target for the treatment of nasopharyngeal carcinoma.

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