Sophoridine suppresses cell growth in human medulloblastoma through FoxM1, NF‑κB and AP‑1

Yue,Zhensong; Si,Tongguo; Pan,Zhanyu; Cao,Wenfeng; Yan,Zhuchen; Jiang,Zhansheng; Ouyang,Huaqiang

doi:10.3892/ol.2017.7224

Sophoridine suppresses cell growth in human medulloblastoma through FoxM1, NF‑κB and AP‑1

Authors:
- Zhensong Yue
- Tongguo Si
- Zhanyu Pan
- Wenfeng Cao
- Zhuchen Yan
- Zhansheng Jiang
- Huaqiang Ouyang
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Affiliations: Department of Integrated Chinese and Western Medicine, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, P.R. China, Department of Invasive Technology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, P.R. China, Department of Pathology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, P.R. China
Published online on: October 19, 2017 https://doi.org/10.3892/ol.2017.7224
Pages: 7941-7946

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Abstract

Sophoridine is an alkaloid extracted from Sophora alopecuroides that has extensive pharmacological actions. In the present study, the effect of sophoridine on cell growth of human medulloblastoma and its mechanism were investigated. Human medulloblastoma D283‑Med cells were incubated with 0, 0.5, 1 or 2 mg/ml sophoridine for 24, 48 or 72 h. Cell proliferation and cytotoxicity were analyzed using MTT and lactate dehydrogenase assays, respectively. Next, analyses of cell apoptosis and caspase‑3/8 activity were performed using flow cytometry or spectrophotometry, respectively. Lastly, the change in FoxM1, TrkB, BDNF, NF‑κB and AP‑1 expression was investigated using western blot analysis. In the present study, treatment with sophoridine significantly suppressed cell growth and induced apoptosis in human medulloblastoma cells. In addition, sophoridine significantly increased cytotoxicity and caspase‑3/8 activity in human medulloblastoma. Finally, it was found that sophoridine suppresses the protein expression of FoxM1, TrkB, BDNF NF‑κB and AP‑1 in human medulloblastoma cells. The present study suggests that sophoridine suppresses cell growth of human medulloblastoma through the inhibition of the FoxM1, NF‑κB and AP‑1 signaling pathway.

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