MicroRNA‑144 inhibits proliferation by targeting WW domain‑containing transcription regulator protein 1 in papillary thyroid cancer

Sun,Wei; Lan,Xiabin; Wang,Zhihong; Dong,Wenwu; He,Liang; Zhang,Ting; Zhang,Ping; Zhang,Hao

doi:10.3892/ol.2017.7440

MicroRNA‑144 inhibits proliferation by targeting WW domain‑containing transcription regulator protein 1 in papillary thyroid cancer

Authors:
- Wei Sun
- Xiabin Lan
- Zhihong Wang
- Wenwu Dong
- Liang He
- Ting Zhang
- Ping Zhang
- Hao Zhang
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Affiliations: Department of Thyroid Surgery, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
Published online on: November 17, 2017 https://doi.org/10.3892/ol.2017.7440
Pages: 1007-1013
Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Papillary thyroid carcinoma (PTC), the most common histological subtype of thyroid cancer, accounts for between 80 and 90% of all thyroid cancer cases. Previous studies have suggested that microRNAs (miRNAs/miRs) are involved in the development of PTC. The aim of the present study was to investigate whether miR‑144 inhibits cellular proliferation in PTC. The expression of miR‑144 was detected in PTC and corresponding adjacent non‑cancerous tissues, and in the PTC cell line IHH4, using reverse transcription‑quantitative polymerase chain reaction. Associations between miR‑144 expression levels and the clinicopathological characteristics were analyzed. Receiver operating characteristic (ROC) curves were used to assess the diagnostic value of miR‑144 expression, and the potential function of miR‑144 was investigated in IHH4 cells using a Cell Counting Kit‑8 and colony formation assays. Western blotting was applied to analyze the expression level of WW domain‑containing transcription regulator 1 (WWTR1) in PTC tissues. miR‑144 was significantly downregulated in PTC tissues and the PTC cell line. Low expression of miR‑144 was associated with larger tumor sizes (P<0.001). The ROC curves demonstrated that miR‑144 may be a potential biomarker for identifying PTC and non‑cancerous diseases (sensitivity, 58.7%; specificity, 87.3%) as well as to differentiate PTC with tumor sizes ≥2 cm (sensitivity, 79.2%; specificity, 69.2%). Upregulation of miR‑144 significantly suppressed proliferation in IHH4 cells. WWTR1 was overexpressed in PTC tissues compared with in adjacent non‑cancerous tissues, and the ectopic expression of miR‑144 downregulated WWTR1 in IHH4 cells. Co‑transfection with pcDNA‑WWTR1 and miR‑144 ‘rescued’ the proliferation inhibition. The results of the present study collectively demonstrated that miR‑144 is downregulated in PTC, that low expression levels of miR‑144 are associated with larger tumor sizes and that miR‑144 inhibits cellular proliferation in PTC by targeting WWTR1.

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