MiR‑370 promotes apoptosis in colon cancer by directly targeting MDM4

Shen,Xiaogang; Zuo,Xiaofei; Zhang,Wenjin; Bai,Yifeng; Qin,Xianpeng; Hou,Nengyi

doi:10.3892/ol.2017.7524

MiR‑370 promotes apoptosis in colon cancer by directly targeting MDM4

Authors:
- Xiaogang Shen
- Xiaofei Zuo
- Wenjin Zhang
- Yifeng Bai
- Xianpeng Qin
- Nengyi Hou
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Affiliations: Department of Gastrointestinal Surgery, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, Sichuan 610041, P.R. China, Department of Clinical Medicine, Chengdu Medical College, Chengdu, Sichuan 610083, P.R. China, Department of Oncology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, Sichuan 610041, P.R. China
Published online on: December 5, 2017 https://doi.org/10.3892/ol.2017.7524
Pages: 1673-1679
Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNA (miR)-370 functions as a tumor suppressor or promoter in several cancers. However, the expression and biological role of miR‑370 in colon cancer remains undefined. In the present study, miR‑370 expression in both normal and malignant colon tissues was quantified by quantitative polymerase chain reaction. An in vitro cell viability and apoptosis assay and an in vitro xenograft tumor model were employed to investigate the role of miR‑370 on colon cancer growth. Furthermore, the potential direct target of miR‑370 was identified using a luciferase assay. Our results demonstrate that down‑regulation of miR‑370 expression occurs in malignant tissues and miR‑370 expression is inversely correlated with tumor grade. Moreover, we determined that miR‑370 functions as a tumor suppressor in colon cancer by inhibiting cell proliferation and promoting cell apoptosis. In addition, overexpression of miR‑370 impairs xenograft tumor growth in nude mice. Mechanistically, mouse double minute 4 (MDM4) was demonstrated to be a potential direct target of miR‑370, inducing apoptosis in colon cancer. Collectively, these findings suggest that upregulation of miR‑370 may impair colon tumor growth by directly targeting MDM4. These findings provide a new direction for the diagnosis and treatment of colon cancer.

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