Upregulation of seladin‑1 and nestin expression in bone marrow mesenchymal stem cell transplantation via the ERK1/2 and PI3K/Akt signaling pathways in an Alzheimer's disease model

  • Authors:
    • Shi Yu
    • Yue Hei
    • Weiping Liu
  • View Affiliations

  • Published online on: December 7, 2017     https://doi.org/10.3892/ol.2017.7543
  • Pages: 7443-7449
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Abstract

The aim of the present study was to determine the roles of bone marrow mesenchymal stem cell (BM‑MSC) transplantation in a model of Alzheimer's disease (AD) and determine the underlying mechanism. The expression of selective Alzheimer's disease indicator‑1 (Seladin‑1) and nestin was detected using reverse transcription‑quantitative polymerase chain reaction and western blot analysis. The phosphoinositide 3‑kinase (PI3K) and extracellular signal‑regulated kinase (ERK)1/2 inhibitors, LY294002 and PD98059, were employed to evaluate the molecular mechanism. The results indicated that the mRNA and protein expression of Seladin‑1 and nestin was lower in the AD group when compared with the control group. BM‑MSC transplantation reversed this decrease in expression, potentially by increasing the protein expression of phosphorylated (p)‑protein kinase B (Akt) and p‑ERK1/2. In addition, LY294002 (the PI3K inhibitor) and/or PD98059 (the ERK1/2 inhibitor) blocked the enhancement of BM‑MSC transplantation on the expression of Seladin‑1 and nestin in the hippocampus. These results indicated that BM‑MSC transplantation enhanced Seladin‑1 and nestin expression potentially via a mechanism associated with the activation of the PI3K/Akt and ERK1/2 signaling pathways. The present study offers preliminary evidence that treatment with BM‑MSCs may represent a potential therapeutic approach against brain lesions in AD.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yu S, Hei Y and Liu W: Upregulation of seladin‑1 and nestin expression in bone marrow mesenchymal stem cell transplantation via the ERK1/2 and PI3K/Akt signaling pathways in an Alzheimer's disease model. Oncol Lett 15: 7443-7449, 2018
APA
Yu, S., Hei, Y., & Liu, W. (2018). Upregulation of seladin‑1 and nestin expression in bone marrow mesenchymal stem cell transplantation via the ERK1/2 and PI3K/Akt signaling pathways in an Alzheimer's disease model. Oncology Letters, 15, 7443-7449. https://doi.org/10.3892/ol.2017.7543
MLA
Yu, S., Hei, Y., Liu, W."Upregulation of seladin‑1 and nestin expression in bone marrow mesenchymal stem cell transplantation via the ERK1/2 and PI3K/Akt signaling pathways in an Alzheimer's disease model". Oncology Letters 15.5 (2018): 7443-7449.
Chicago
Yu, S., Hei, Y., Liu, W."Upregulation of seladin‑1 and nestin expression in bone marrow mesenchymal stem cell transplantation via the ERK1/2 and PI3K/Akt signaling pathways in an Alzheimer's disease model". Oncology Letters 15, no. 5 (2018): 7443-7449. https://doi.org/10.3892/ol.2017.7543