Celastrol suppresses the proliferation of lung adenocarcinoma cells by regulating microRNA-24 and microRNA-181b

Yan,Yun‑Fei; Zhang,Han‑Han; Lv,Qing; Liu,Yue‑Mei; Li,You‑Jie; Li,Bao‑Sheng; Wang,Ping‑Yu; Shang,Wen‑Jing; Yue,Zhen; Xie,Shu‑Yang

doi:10.3892/ol.2017.7593

Celastrol suppresses the proliferation of lung adenocarcinoma cells by regulating microRNA-24 and microRNA-181b

Authors:
- Yun‑Fei Yan
- Han‑Han Zhang
- Qing Lv
- Yue‑Mei Liu
- You‑Jie Li
- Bao‑Sheng Li
- Ping‑Yu Wang
- Wen‑Jing Shang
- Zhen Yue
- Shu‑Yang Xie
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Affiliations: Department of Biochemistry and Molecular Biology, Key Laboratory of Tumor Molecular Biology, Binzhou Medical University, Yantai, Shandong 264003, P.R. China, Department of Radiation Oncology, Shandong Cancer Hospital, Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China
Published online on: December 12, 2017 https://doi.org/10.3892/ol.2017.7593
Pages: 2515-2521

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Abstract

Cumulative evidence has indicated that celastrol may suppress cancer growth; however, the underlying mechanism requires further investigation. In the present study, A549 cells were treated with various concentrations of celastrol. Lung cancer cell proliferation was evaluated using an MTT assay and observed under a microscope. Cell apoptosis was detected by Annexin V fluorescein isothiocyanate/propidium iodide double‑labeled flow cytometry. The results demonstrated that celastrol suppressed proliferation and induced apoptosis in a dose‑independent manner. Celastrol may also decrease the phosphorylation levels of signal transducer and activator of transcription 3 (STAT3) and the B cell lymphoma‑2 (Bcl‑2)/Bcl‑2 associated C protein (Bax) ratio. As microRNA (miR‑24 and miR‑181b) were predicated to target STAT3, STAT3 activation was inhibited in miR‑24‑or miR‑181b‑treated A549 cells compared with the control treatment. The ratio of Bcl‑2/Bax was further reduced in miR‑24 or miR‑181b‑treated A549 cells. The results were further confirmed by detecting in another lung adenocarcinoma cell line, LTEP‑a‑2. In summary, the results of the present study demonstrated that celastrol treatment suppressed the proliferation and induced apoptosis by regulating the expression levels of miR‑24 and miR‑181b.

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