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mir‑106a regulates cell proliferation and apoptosis of colon cancer cells through targeting the PTEN/PI3K/AKT signaling pathway

  • Authors:
    • Yan Qin
    • Zhibin Huo
    • Xiang Song
    • Xiao Chen
    • Xiaopeng Tian
    • Xinjie Wang

  • View Affiliations

  • Published online on: December 29, 2017     https://doi.org/10.3892/ol.2017.7715
  • Pages: 3197-3201
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Abstract

Colorectal cancer (CRC) is a common digestive tract tumor. Cancer tissues and healthy tissues were extracted from patients with CRC who were treated at our hospital. Targetscan and PicTar were used to identify microRNAs (miRNAs/miRs) that may interact with phosphatase and tensin homolog deleted on chromosome ten (PTEN). Dual luciferase reporter assay was applied to detect whether the 3'‑untranslated region (UTR) of PTEN was targeted by miR‑106a. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) showed that there was significantly higher miR‑106a expression level in cancer tissue compared with in healthy tissue. The expression level of miR‑106a in NCM640, SW620 and HT29 cell lines was detected by RT‑qPCR, and HT29 cells showed the highest miR‑106a level. HT29 cells were used for the present study, separated into control, miR‑NC antagomiR and miR‑106a antagomiR group. HT29 cell characteristics were tested. The results demonstrated that in the miR‑106a antagomiR group, there was a lower cell proliferation and higher cell apoptosis rate compared with the control and miR‑NC antagomiR groups. miR‑106a was verified to target PTEN 3'‑UTR in HT29 cells. In comparison with control and miR‑NC antagomiR groups, the protein level of PTEN was increased and phosphatidylinositol‑4,5‑bisphosphate 3‑kinase/protein kinase B was decreased following miR‑766 antagomiR administration. The findings propose that miR‑106a may serve a therapeutic target for the treatment of CRC.
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March-2018
Volume 15 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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  • CiteScore: 5.7
  • CiteScore Rank: Q2: #139/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.574
  • SCImago Journal Rank (SJR): 0.644
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Spandidos Publications style
Qin Y, Huo Z, Song X, Chen X, Tian X and Wang X: mir‑106a regulates cell proliferation and apoptosis of colon cancer cells through targeting the PTEN/PI3K/AKT signaling pathway. Oncol Lett 15: 3197-3201, 2018.
APA
Qin, Y., Huo, Z., Song, X., Chen, X., Tian, X., & Wang, X. (2018). mir‑106a regulates cell proliferation and apoptosis of colon cancer cells through targeting the PTEN/PI3K/AKT signaling pathway. Oncology Letters, 15, 3197-3201. https://doi.org/10.3892/ol.2017.7715
MLA
Qin, Y., Huo, Z., Song, X., Chen, X., Tian, X., Wang, X."mir‑106a regulates cell proliferation and apoptosis of colon cancer cells through targeting the PTEN/PI3K/AKT signaling pathway". Oncology Letters 15.3 (2018): 3197-3201.
Chicago
Qin, Y., Huo, Z., Song, X., Chen, X., Tian, X., Wang, X."mir‑106a regulates cell proliferation and apoptosis of colon cancer cells through targeting the PTEN/PI3K/AKT signaling pathway". Oncology Letters 15, no. 3 (2018): 3197-3201. https://doi.org/10.3892/ol.2017.7715