MicroRNA‑125a‑5p inhibits invasion and metastasis of gastric cancer cells by targeting BRMS1 expression

Cao,Yi; Tan,Shengxing; Tu,Yi; Zhang,Guoyang; Liu,Yi; Li,Daojiang; Xu,Shan; Le,Zhibiao; Xiong,Jianbo; Zou,Wenyu; Gong,Peitao; Li,Zhengrong; Jie,Zhigang

doi:10.3892/ol.2018.7983

MicroRNA‑125a‑5p inhibits invasion and metastasis of gastric cancer cells by targeting BRMS1 expression

Authors:
- Yi Cao
- Shengxing Tan
- Yi Tu
- Guoyang Zhang
- Yi Liu
- Daojiang Li
- Shan Xu
- Zhibiao Le
- Jianbo Xiong
- Wenyu Zou
- Peitao Gong
- Zhengrong Li
- Zhigang Jie
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Affiliations: Department of General Surgery, First Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Pathology, First Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330006, P.R. China
Published online on: February 7, 2018 https://doi.org/10.3892/ol.2018.7983
Pages: 5119-5130

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Abstract

Accumulating studies have demonstrated microRNAs (miRNAs/miRs) have an important role in multiple processes of human malignant tumor development and progression. Decreased expression of miR‑125a‑5p has been observed in several types of cancer, including gastric cancer (GC). However, the mechanism and exact function of miR‑125a‑5p in GC have not been largely elucidated. In the present study, reverse transcription‑quantitative polymerase chain reaction indicated that the expression of miR‑125a‑5p was downregulated in GC tissues and cell lines compared with matched normal tissues (P<0.01) and normal gastric mucosa cell lines (P<0.01), respectively. Moreover, clinical pathological characteristics and Kaplan‑Meier analysis indicated that a low expression of miR‑125a‑5p was not only associated with lymph metastasis, peritoneal dissemination and advanced tumor‑node metastasis stage but also affected the prognosis of GC patients. Compared with miR‑control‑transfected GC cells, markedly decreased migration and invasion was observed in GC cells that overexpress miR‑125a‑5p. By contrast, increased metastasis and invasion were observed in miR‑125a‑5p‑knocked down cells compared with the control. Furthermore, luciferase reporter assays indicated that breast cancer metastasis suppressor 1 (BRMS1) was a direct target of miR‑125a‑5p. Notably, a positive correlation between the levels of BRMS1 and miR‑125a‑5p in GC tissues was observed, and BRMS1 expression was indicated to be regulated by miR‑125a‑5p in GC cells. In conclusion, miR‑125a‑5p may act as a tumor suppressor by targeting the metastasis‑inhibitory gene, BRMS1. The data suggesting that BRMS1 is a potential target gene of miR‑125a‑5p, may provide novel insight into miRNA regulation of human gene expression, and a useful target for gene therapy of GC.

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