Hydroxycamptothecin mediates antiproliferative effects through apoptosis and autophagy in A549 cells

Wei,Yanjie; Li,Chenhao; Zhang,Yuan; He,Hailan; Zhang,Guozhi; Hao,Xiaohui; Liu,Heliang; Wang,Hongli; Tian,Wei

doi:10.3892/ol.2018.8107

Hydroxycamptothecin mediates antiproliferative effects through apoptosis and autophagy in A549 cells

Authors:
- Yanjie Wei
- Chenhao Li
- Yuan Zhang
- Hailan He
- Guozhi Zhang
- Xiaohui Hao
- Heliang Liu
- Hongli Wang
- Wei Tian
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Affiliations: Medical Research Center, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China, Department of Oncology, Cangzhou Central Hospital, Cangzhou, Hebei 061000, P.R. China, Department of General Surgery, North China University of Science and Technology Affiliated Hospital, Tangshan, Hebei 063000, P.R. China
Published online on: February 22, 2018 https://doi.org/10.3892/ol.2018.8107
Pages: 6322-6328
Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hydroxycamptothecin (HCPT) represents a new generation of anticancer drugs, with almost no side effects when used for the treatment of a number of types of cancer. Autophagy is becoming recognized as an important biological mechanism in human cancer, including lung cancer. However, the involvement of autophagy in the antiproliferative effects of HCPT on lung cancer remains unclear. In the present study, A549 cells, an accepted model of non‑small cell lung cancer (NSCLC) cells, were employed. It was demonstrated that HCPT was able to suppress proliferation and induce apoptosis and autophagy in A549 cells. The molecular mechanism underlying HCPT‑induced cell death was attributed to apoptosis and autophagy. Furthermore, it was demonstrated that an autophagy inhibitor, 3‑methyladenine, accelerated HCPT‑induced cell death in A549 cells. The results of the present study may lead to a deeper understanding of the molecular mechanism by which HCPT regulates NSCLC A549 cells. These results highlight the potential use of autophagy inhibitors in combination with traditional chemotherapy drugs for the treatment of lung cancer.

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