Plasmodium circumsporozoite protein suppresses the growth of A549 cells via inhibiting nuclear transcription factor κB

Deng,Xu‑Feng; Zhou,Dong; Liu,Quan‑Xing; Zheng,Hong; Ding,Yan; Xu,Wen‑Yue; Min,Jia‑Xin; Dai,Ji‑Gang

doi:10.3892/ol.2018.8115

Plasmodium circumsporozoite protein suppresses the growth of A549 cells via inhibiting nuclear transcription factor κB

Authors:
- Xu‑Feng Deng
- Dong Zhou
- Quan‑Xing Liu
- Hong Zheng
- Yan Ding
- Wen‑Yue Xu
- Jia‑Xin Min
- Ji‑Gang Dai
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Affiliations: Department of Thoracic Surgery, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, P.R. China, Institute of Immunology of PLA, College of Basic Medical Sciences, The Third Military Medical University, Chongqing 400038, P.R. China, Department of Pathogenic Biology, College of Basic Medical Sciences, The Third Military Medical University, Chongqing 400038, P.R. China
Published online on: February 26, 2018 https://doi.org/10.3892/ol.2018.8115
Pages: 6585-6591

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Abstract

Blocking the activation of nuclear factor κB (NF-κB) is a promising strategy for the treatment of non-small cell lung cancer. The circumsporozoite protein (CSP), a key component of the sporozoite stage of the malaria parasite, was previously reported to block NF‑κB activation in hepatocytes. Therefore, in the present study, the effect of CSP on the growth of the human lung cancer cell line, A549, was investigated. It was demonstrated that transfection with a recombinant plasmid expressing CSP was able to inhibit the proliferation of A549 cells in a dose‑dependent manner and induce the apoptosis of A549 cells. A NF‑κB gene reporter assay indicated that CSP and its nuclear localization signal (NLS) motif were able to equally suppress the activation of NF‑κB following stimulation with human recombinant tumor necrosis factor (TNF)‑α in A549 cells. Furthermore, western blot analysis indicated that NLS did not affect the phosphorylation and degradation of IκB, but was able to markedly inhibit the nuclear translocation of NF-κB in TNF-α stimulated A549 cells. Therefore, the data suggest that CSP may be investigated as a potential novel NF‑κB inhibitor for the treatment of lung cancer.

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