Effects of DKK1 overexpression on bone metastasis of SBC-3 cells

Pang,Hailin; Ma,Ningqiang; Shen,Weiwei; Zhao,Qiang; Wang,Jianlin; Duan,Lian; Chen,Wenjuan; Zhang,Ning; Zhao,Zhengwei; Liu,Lili; Zhang,Helong

doi:10.3892/ol.2018.8160

Effects of DKK1 overexpression on bone metastasis of SBC-3 cells

Authors:
- Hailin Pang
- Ningqiang Ma
- Weiwei Shen
- Qiang Zhao
- Jianlin Wang
- Lian Duan
- Wenjuan Chen
- Ning Zhang
- Zhengwei Zhao
- Lili Liu
- Helong Zhang
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Affiliations: Department of Oncology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China, Department of Oncology, The Third Hospital of PLA, Baoji, Shaanxi 721004, P.R. China, Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710038, P.R. China
Published online on: March 2, 2018 https://doi.org/10.3892/ol.2018.8160
Pages: 6739-6744

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Abstract

Among all malignancies, lung cancer is the leading cause of cancer-related deaths in China. Bone metastasis is one of the most common complications and one of the most important factors affecting the prognosis of lung cancer patients, which resulting in very poor therapeutic effects. Previously, we have demonstrated that the expression levels of Dickkopf1 (DKK1), a protein involved in cell regulation and proliferation, was dramatically higher in cells that have a tendency to metastasize and invade the bone tissue (SBC‑5 cells) compared with cells that do not (SBC‑3 cells). Downregulation of DKK1 in SBC‑5 cells inhibited cell malignancy in vitro, and the formation of bone metastasis in vivo. However, whether upregulating DKK1 would be sufficient to induce aggressive tumor behavior (proliferation, migration, invasion and metastasis) in SBC‑3 cells remained to be investigated. The present study aimed to examine the role of DKK1 in SBC‑3 cells, as well as to investigate the SBC‑3 ability to metastasize and invade the bone tissue. The results demonstrated that upregulation of DKK1 in SBC‑3 cells enhanced cell proliferation, colony formation, cell migration and invasion in vitro, as well as bone metastasis in vivo. These results indicate that DKK1 may be an important regulator in the development of small cell lung cancer (SCLC), and targeting DKK1 may be an effective method for preventing and/or treating skeletal metastases in SCLC cases.

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