Effect of photodynamic therapy combined with Celecoxib on expression of cyclooxygenase-2 protein in HeLa cells

  • Authors:
    • Yuanfu Mao
    • Lishuang Wang
    • Chen Xu
    • Shiyu Han
  • View Affiliations

  • Published online on: March 2, 2018     https://doi.org/10.3892/ol.2018.8163
  • Pages: 6599-6603
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Abstract

The present study assessed the effect of photodynamic therapy (PDT) combined with Celecoxib (Cel) on cervical cancer HeLa cells. An MTT assay was performed to detect the inhibitory effects of Cel with different concentrations (10, 50, 100, 150, 200, 250 and 300 µg/ml) on the proliferation of HeLa cells. Subsequently, HeLa cells were divided into control group (group H), 50 g/ml Celecoxib group (group C), PDT group (group P), 50 g/ml Cel + PDT group (group P + C) and western blotting and immunohistochemistry were performed to detect the expression of cyclooxygenase‑2 (COX‑2) protein in the different groups. Cel inhibited HeLa cells proliferation 24 h following administration, among which 200 µg/ml induced a 50% inhibition rate; the relative expression level of COX‑2 protein in group P + C was significantly decreased compared with that in either group C or group P (P<0.05). Cel inhibited the proliferation of human cervical cancer cells in a concentration-dependent manner, and combined PDT therapy may improve treatment outcomes.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Mao Y, Wang L, Xu C and Han S: Effect of photodynamic therapy combined with Celecoxib on expression of cyclooxygenase-2 protein in HeLa cells. Oncol Lett 15: 6599-6603, 2018
APA
Mao, Y., Wang, L., Xu, C., & Han, S. (2018). Effect of photodynamic therapy combined with Celecoxib on expression of cyclooxygenase-2 protein in HeLa cells. Oncology Letters, 15, 6599-6603. https://doi.org/10.3892/ol.2018.8163
MLA
Mao, Y., Wang, L., Xu, C., Han, S."Effect of photodynamic therapy combined with Celecoxib on expression of cyclooxygenase-2 protein in HeLa cells". Oncology Letters 15.5 (2018): 6599-6603.
Chicago
Mao, Y., Wang, L., Xu, C., Han, S."Effect of photodynamic therapy combined with Celecoxib on expression of cyclooxygenase-2 protein in HeLa cells". Oncology Letters 15, no. 5 (2018): 6599-6603. https://doi.org/10.3892/ol.2018.8163