Expression of Caspase-1 in breast cancer tissues and its effects on cell proliferation, apoptosis and invasion

Sun,Yanxia; Guo,Yingzhen

doi:10.3892/ol.2018.8176

Expression of Caspase-1 in breast cancer tissues and its effects on cell proliferation, apoptosis and invasion

Authors:
- Yanxia Sun
- Yingzhen Guo
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Affiliations: Department of Galactophore, The First People's Hospital of Xinxiang, Xinxiang, Henan 453000, P.R. China
Published online on: March 5, 2018 https://doi.org/10.3892/ol.2018.8176
Pages: 6431-6435
Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to detect the expression of Caspase-1 in the tumor tissues and tumor‑adjacent tissues of patients with breast cancer, and to investigate the effects of Caspase‑1 on the proliferation, apoptosis and invasion of breast cancer cells. Reverse transcription‑quantitative polymerase chain reaction was used to detect Caspase‑1 mRNA expression in breast cancer tissues and tumor‑adjacent tissues from patients. Additionally, the human breast cancer MDA‑MB‑231 cell line was treated with the Caspase‑1 small molecule inhibitor Ac‑YVAD‑CMK, following which the changes to Caspase‑1 protein expression were detected via western blotting. The MTT method detected the changes to cell proliferation, flow cytometry detected the rate of apoptosis, and a Transwell assay was employed to assess invasion. Caspase‑1 mRNA expression was significantly decreased in the breast cancer tissues of patients, compared with in the tumor‑adjacent tissues, a difference that was statistically significant (P<0.05). Treatment with the Ac‑YVAD‑CMK markedly decreased the protein expression of Caspase‑1 in MDA‑MB‑231 cells, and the difference was statistically significant (P<0.05). Following this treatment of Ac‑YVAD‑CMK cells, the proliferation and invasion abilities markedly increased, while the apoptotic levels significantly decreased (P<0.05). In conclusion, the expression of Caspase‑1 is low in breast cancer tissues, which may promote the proliferation and invasion of breast cancer cells and could be closely associated with the occurrence and development of breast cancer.

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