Open Access

Therapeutic role of meloxicam targeting secretory clusterin-mediated invasion in hepatocellular carcinoma cells

  • Authors:
    • Jingtao Zhong
    • Xiaoming Yu
    • Xiaofeng Dong
    • Hong Lu
    • Wuyuan Zhou
    • Lei Li
    • Zhongchao Li
    • Pengfei Sun
    • Xuetao Shi
  • View Affiliations

  • Published online on: March 7, 2018     https://doi.org/10.3892/ol.2018.8186
  • Pages: 7191-7199
  • Copyright: © Zhong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Recurrence and metastasis are the two leading causes of poor prognosis in patients with hepatocellular carcinoma (HCC). Secreted clusterin (sCLU) is a stress‑induced chaperone that is overexpressed in HCC. However, the precise molecular mechanisms of sCLU in HCC invasion and migration are largely unknown. In the present study, it was indicated that downregulation of sCLU significantly alleviated invasiveness whereas overexpression of sCLU notably enhanced the number of invasive cells via mediating the expression level of MMP‑2 and E‑cadherin in Bel‑7402 and SMMC‑7721 cells. Furthermore, as an important mediator of invasiveness, sCLU may be responsible for proliferation and invasion suppression induced by meloxicam (a selective inhibitor of cyclooxygenase‑2) in HCC cells. The combination of meloxicam and CLU shRNA significantly decreased invasion in HCC cells in vitro. Furthermore, it was observed that overexpression of sCLU significantly potentiated expression of p‑AKT and MMP‑2. However, downregulation of sCLU by CLU shRNA alleviated the extent of p‑AKT. These results suggest the targeting of sCLU may be a novel therapeutic strategy against invasion and migration in HCC.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Zhong J, Yu X, Dong X, Lu H, Zhou W, Li L, Li Z, Sun P and Shi X: Therapeutic role of meloxicam targeting secretory clusterin-mediated invasion in hepatocellular carcinoma cells. Oncol Lett 15: 7191-7199, 2018
APA
Zhong, J., Yu, X., Dong, X., Lu, H., Zhou, W., Li, L. ... Shi, X. (2018). Therapeutic role of meloxicam targeting secretory clusterin-mediated invasion in hepatocellular carcinoma cells. Oncology Letters, 15, 7191-7199. https://doi.org/10.3892/ol.2018.8186
MLA
Zhong, J., Yu, X., Dong, X., Lu, H., Zhou, W., Li, L., Li, Z., Sun, P., Shi, X."Therapeutic role of meloxicam targeting secretory clusterin-mediated invasion in hepatocellular carcinoma cells". Oncology Letters 15.5 (2018): 7191-7199.
Chicago
Zhong, J., Yu, X., Dong, X., Lu, H., Zhou, W., Li, L., Li, Z., Sun, P., Shi, X."Therapeutic role of meloxicam targeting secretory clusterin-mediated invasion in hepatocellular carcinoma cells". Oncology Letters 15, no. 5 (2018): 7191-7199. https://doi.org/10.3892/ol.2018.8186