Tannic acid mediated induction of apoptosis in human glioma Hs 683 cells

Zhang,Jian; Chen,Dong; Han,Dian‑Ming; Cheng,Yan‑Hao; Dai,Chao; Wu,Xiu‑Jie; Che,Feng‑Yuan; Heng,Xue‑Yuan

doi:10.3892/ol.2018.8197

Tannic acid mediated induction of apoptosis in human glioma Hs 683 cells

Authors:
- Jian Zhang
- Dong Chen
- Dian‑Ming Han
- Yan‑Hao Cheng
- Chao Dai
- Xiu‑Jie Wu
- Feng‑Yuan Che
- Xue‑Yuan Heng
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Affiliations: Department of Neurosurgery, Linyi People's Hospital, Linyi, Shandong 276000, P.R. China, Department of Clinical College, Binzhou Medical University, Yantai, Shandong 264000, P.R. China, Department of Neurology, Linyi People's Hospital, Linyi, Shandong 276000, P.R. China
Published online on: March 7, 2018 https://doi.org/10.3892/ol.2018.8197
Pages: 6845-6850
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Tannic acid (TA), a natural plant compound, is known to induce the death of cancer cells in various types of cancer. The present study was designed with the aim of exploring the effects of tannic acid in vitro on HS 683, a glioma cell line, and to study the mechanism involved in the induction of cytotoxicity and apoptosis by TA. TA exhibited maximum cytotoxic activity against the Hs 683 cell line. Nuclear morphology, 4',6‑diamidino‑2‑phenylindole staining and annexin V/propidium iodide apoptosis assaying of Hs 683 cells confirmed that cell death was due to the induction of apoptosis by TA. Further mechanistic study of TA on Hs 683 cells revealed that it decreased cell growth with increasing TA concentration, that resulted in the activation of pro‑caspase 3 and caspase 9 and the cleavage of poly (ADP‑ribose) polymerase, implying the induction of apoptosis cascades. Biochemical evidence of apoptosis resulted from the loss of mitochondrial membrane potential and increased intracellular reactive oxygen species production by TA in a dose‑dependent manner. Based on this data, TA may be further investigated as a potential anticancer therapeutic lead.

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1	Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI
2	Louis DN, Ohgaki H, Wiestler OD, Cavenee WK, Burger PC, Jouvet A, Scheithauer BW and Kleihues P: The 2007 WHO classification of tumors of the central nervous system. Acta Neuropathol. 114:97–109. 2007. View Article : Google Scholar : PubMed/NCBI
3	Fuller GN: The WHO classification of tumours of the central nervous system. 4th edition. Arch Pathol Lab Med. 132:9062008.PubMed/NCBI
4	Rock K, McArdle O, Forde P, Dunne M, Fitzpatrick D, O'Neill B and Faul C: A clinical review of treatment outcomes in glioblastoma multiforme-the validation in a non-trial population of the results of a randomised Phase III clinical trial: Has a more radical approach improved survival? Br J Radiol. 85:e729–e733. 2012. View Article : Google Scholar : PubMed/NCBI
5	Singh S, Sharma B, Kanwar SS and Kumar A: Lead phytochemicals for anticancer drug development. Front Plant Sci. 7:16672016. View Article : Google Scholar : PubMed/NCBI
6	Rahman MA, Amin AR and Shin DM: Chemopreventive potential of natural compounds in head and neck cancer. Nutr Cancer. 62:973–987. 2010. View Article : Google Scholar : PubMed/NCBI
7	D'Alessandro N, Poma P and Montalto G: Multifactorial nature of hepatocellular carcinoma drug resistance: Could plant polyphenols be helpful? World J Gastroenterol. 13:2037–2043. 2007. View Article : Google Scholar : PubMed/NCBI
8	Rudolf E, Andelová H and Cervinka M: Polyphenolic compounds in chemoprevention of colon cancer-targets and signaling pathways. Anticancer Agents Med Chem. 7:559–575. 2007. View Article : Google Scholar : PubMed/NCBI
9	Stevenson DE and Hurst RD: Polyphenolic phytochemicals-just antioxidants or much more? Cell Mol Life Sci. 64:2900–2916. 2007. View Article : Google Scholar : PubMed/NCBI
10	Fresco P, Borges F, Diniz C and Marques MP: New insights on the anticancer properties of dietary polyphenols. Med Res Rev. 26:747–766. 2006. View Article : Google Scholar : PubMed/NCBI
11	Romani A, Ieri F, Turchetti B, Mulinacci N, Vincieri FF and Buzzini P: Analysis of condensed and hydrolysable tannins from commercial plant extracts. J Pharm Biomed Anal. 41:415–420. 2006. View Article : Google Scholar : PubMed/NCBI
12	Gali-Muhtasib HU, Yamout SZ and Sidani MM: Tannins protect against skin tumor promotion induced by ultraviolet-B radiation in hairless mice. Nutr Cancer. 37:73–77. 2000. View Article : Google Scholar : PubMed/NCBI
13	Sakagami H, Jiang Y, Kusama K, Atsumi T, Ueha T, Toguchi M, Iwakura I, Satoh K, Ito H, Hatano T and Yoshida T: Cytotoxic activity of hydrolyzable tannins against human oral tumor cell lines-a possible mechanism. Phytomedicine. 7:39–47. 2000. View Article : Google Scholar : PubMed/NCBI
14	Pan MH, Lin JH, Lin-Shiau SY and Lin JK: Induction of apoptosis by penta-O-galloyl-beta-D-glucose through activation of caspase-3 in human leukemia HL-60 cells. Eur J Pharmacol. 381:171–183. 1999. View Article : Google Scholar : PubMed/NCBI
15	Wang CC, Chen LG and Yang LL: Cuphiin D1, the macrocyclic hydrolyzable tannin induced apoptosis in HL-60 cell line. Cancer Lett. 149:77–83. 2000. View Article : Google Scholar : PubMed/NCBI
16	Yang LL, Lee CY and Yen KY: Induction of apoptosis by hydrolyzable tannins from Eugenia jambos L. on human leukemia cells. Cancer Lett. 157:65–75. 2000. View Article : Google Scholar : PubMed/NCBI
17	Nam S, Smith DM and Dou QP: Tannic acid potently inhibits tumor cell proteasome activity, increases p27 and Bax expression, and induces G1 arrest and apoptosis. Cancer Epidemiol Biomarkers Prev. 10:1083–8. 2001.PubMed/NCBI
18	Blacher E, Levy A, Baruch BB, Green KD, Garneau-Tsodikova S, Fridman M and Stein R: Targeting CD38 in the tumor microenvironment: A novel approach to treat glioma. Cancer Cell Microenvironment. 2:22015.
19	Lavrik IN, Golks A and Krammer PH: Caspases: Pharmacological manipulation of cell death. J Clin Invest. 115:2665–2672. 2005. View Article : Google Scholar : PubMed/NCBI
20	Sakahira H, Enari M and Nagata S: Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature. 391:96–99. 1998. View Article : Google Scholar : PubMed/NCBI
21	Daly JS and Cooney DO: Interference by tannic acid with the effectiveness of activated charcoal in ‘Universal Antidote’. Clin Toxicol. 12:515–522. 1978. View Article : Google Scholar : PubMed/NCBI
22	Hupkens P, Boxma H and Dokter J: Tannic acid as a topical agent in burns: Historical considerations and implications for new developments. Burns. 21:57–61. 1995. View Article : Google Scholar : PubMed/NCBI
23	Shi-Tsi S: Use of combined traditional Chinese and Western medicine in the management of burns. Panminerva Med. 25:197–202. 1983.PubMed/NCBI
24	Mala A and Tulika T: Therapeutic efficacy of Centella asiatica (L.) and Momordica charantia: As traditional medicinal plant. J Plant Sci. 3:1–9. 2015.
25	Naus PJ, Henson R, Bleeker G, Wehbe H, Meng F and Patel T: Tannic acid synergizes the cytotoxicity of chemotherapeutic drugs in human cholangiocarcinoma by modulating drug efflux pathways. J Hepatol. 46:222–229. 2007. View Article : Google Scholar : PubMed/NCBI
26	Ramanathan R, Tan CH and Das NP: Cytotoxic effect of plant polyphenols and fat-soluble vitamins on malignant human cultured cells. Cancer Lett. 62:217–224. 1992. View Article : Google Scholar : PubMed/NCBI
27	Booth BW, Inskeep BD, Shah H, Park JP, Hay EJ and Burg KJ: Tannic acid preferentially targets estrogen receptor-positive breast cancer. Int J Breast Cancer. 2013:3696092013. View Article : Google Scholar : PubMed/NCBI