Open Access

Tannic acid mediated induction of apoptosis in human glioma Hs 683 cells

  • Authors:
    • Jian Zhang
    • Dong Chen
    • Dian‑Ming Han
    • Yan‑Hao Cheng
    • Chao Dai
    • Xiu‑Jie Wu
    • Feng‑Yuan Che
    • Xue‑Yuan Heng
  • View Affiliations

  • Published online on: March 7, 2018     https://doi.org/10.3892/ol.2018.8197
  • Pages: 6845-6850
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Tannic acid (TA), a natural plant compound, is known to induce the death of cancer cells in various types of cancer. The present study was designed with the aim of exploring the effects of tannic acid in vitro on HS 683, a glioma cell line, and to study the mechanism involved in the induction of cytotoxicity and apoptosis by TA. TA exhibited maximum cytotoxic activity against the Hs 683 cell line. Nuclear morphology, 4',6‑diamidino‑2‑phenylindole staining and annexin V/propidium iodide apoptosis assaying of Hs 683 cells confirmed that cell death was due to the induction of apoptosis by TA. Further mechanistic study of TA on Hs 683 cells revealed that it decreased cell growth with increasing TA concentration, that resulted in the activation of pro‑caspase 3 and caspase 9 and the cleavage of poly (ADP‑ribose) polymerase, implying the induction of apoptosis cascades. Biochemical evidence of apoptosis resulted from the loss of mitochondrial membrane potential and increased intracellular reactive oxygen species production by TA in a dose‑dependent manner. Based on this data, TA may be further investigated as a potential anticancer therapeutic lead.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang J, Chen D, Han DM, Cheng YH, Dai C, Wu XJ, Che FY and Heng XY: Tannic acid mediated induction of apoptosis in human glioma Hs 683 cells. Oncol Lett 15: 6845-6850, 2018
APA
Zhang, J., Chen, D., Han, D., Cheng, Y., Dai, C., Wu, X. ... Heng, X. (2018). Tannic acid mediated induction of apoptosis in human glioma Hs 683 cells. Oncology Letters, 15, 6845-6850. https://doi.org/10.3892/ol.2018.8197
MLA
Zhang, J., Chen, D., Han, D., Cheng, Y., Dai, C., Wu, X., Che, F., Heng, X."Tannic acid mediated induction of apoptosis in human glioma Hs 683 cells". Oncology Letters 15.5 (2018): 6845-6850.
Chicago
Zhang, J., Chen, D., Han, D., Cheng, Y., Dai, C., Wu, X., Che, F., Heng, X."Tannic acid mediated induction of apoptosis in human glioma Hs 683 cells". Oncology Letters 15, no. 5 (2018): 6845-6850. https://doi.org/10.3892/ol.2018.8197