Expression profile of NF-κB regulated genes in sporadic colorectal cancer patients

  • Authors:
    • Betsy Annel González‑Quezada
    • Uriel Francisco Santana‑Bejarano
    • Alfredo Corona‑Rivera
    • Helia Judith Pimentel‑Gutiérrez
    • Rocío Silva‑Cruz
    • Citlalli Ortega‑de-la-Torre
    • Ramón Franco‑Topete
    • Karina Franco‑Topete
    • Manuel Willebaldo Centeno‑Flores
    • Víctor Manuel Maciel‑Gutiérrez
    • Jorge Román Corona‑Rivera
    • Juan Armendáriz‑Borunda
    • Lucina Bobadilla‑Morales
  • View Affiliations

  • Published online on: March 7, 2018     https://doi.org/10.3892/ol.2018.8201
  • Pages: 7344-7354
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Colorectal cancer (CRC) is the fourth leading worldwide cause of cancer‑associated mortalities. Nuclear factor‑κB (NF‑κB) is a transcriptional regulator of multiple genes associated with CRC. Tumor tissue were compared with normal adjacent mucosa from 30 sporadic patients with CRC were investigated. A total of 8 non‑CRC patients were analyzed as a control group. In the present study, the protein expression of NF‑κB/p65 was detected by immunohistochemistry, and the gene expression profiles of cyclin D1 (CCND1), prostaglandin‑endoperoxide synthase 2, vascular endothelial growth factor A, matrix metallopeptidase 9, BCL2 apoptosis regulator (BCL2), BCL2 like 1, nitric oxide synthase 2, tumor necrosis factor and arachidonate lipoxygenase were detected by reverse transcription‑quantitative polymerase chain reaction. NF‑κB/p65 and genes expression profiles were classified according to tumor-node-metastasis (TNM) clinicopathological parameters, followed by statistical analysis. Higher protein expression of NF‑κB/p65 in the cytoplasm of tumor tissues compared with adjacent normal mucosa was reported; this increment was positively associated with all clinicopathological parameters, except for tumor localization site. The selected genes demonstrated a diverse associative pattern when analyzed with clinicopathological parameters. CCND1 was positively associated with all TNM parameters and BCL2 was negatively associated with all TNM parameters, thus indicating their importance as strong molecular biomarkers for CRC. According to these results, not all selected genes regulated by NF‑κB/p65 show increased expression during CRC development, whereas the transcription factor did. The present study suggests that NF‑κB/p65 overexpression is necessary for CRC establishment and progression, but its transcriptional activity is not sufficient to regulate all target genes in CRC. NF‑κB/p65 and the gene expression profiles reported in the present study may be therapeutically useful. Considering the heterogeneity of the disease, the particular evaluation of these molecules may allow for the selection of proper diagnosis, treatment and follow‑up for patients with sporadic CRC.
View Figures
View References

Related Articles

Journal Cover

May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
González‑Quezada BA, Santana‑Bejarano UF, Corona‑Rivera A, Pimentel‑Gutiérrez HJ, Silva‑Cruz R, Ortega‑de-la-Torre C, Franco‑Topete R, Franco‑Topete K, Centeno‑Flores MW, Maciel‑Gutiérrez VM, Maciel‑Gutiérrez VM, et al: Expression profile of NF-κB regulated genes in sporadic colorectal cancer patients. Oncol Lett 15: 7344-7354, 2018
APA
González‑Quezada, B.A., Santana‑Bejarano, U.F., Corona‑Rivera, A., Pimentel‑Gutiérrez, H.J., Silva‑Cruz, R., Ortega‑de-la-Torre, C. ... Bobadilla‑Morales, L. (2018). Expression profile of NF-κB regulated genes in sporadic colorectal cancer patients. Oncology Letters, 15, 7344-7354. https://doi.org/10.3892/ol.2018.8201
MLA
González‑Quezada, B. A., Santana‑Bejarano, U. F., Corona‑Rivera, A., Pimentel‑Gutiérrez, H. J., Silva‑Cruz, R., Ortega‑de-la-Torre, C., Franco‑Topete, R., Franco‑Topete, K., Centeno‑Flores, M. W., Maciel‑Gutiérrez, V. M., Corona‑Rivera, J. R., Armendáriz‑Borunda, J., Bobadilla‑Morales, L."Expression profile of NF-κB regulated genes in sporadic colorectal cancer patients". Oncology Letters 15.5 (2018): 7344-7354.
Chicago
González‑Quezada, B. A., Santana‑Bejarano, U. F., Corona‑Rivera, A., Pimentel‑Gutiérrez, H. J., Silva‑Cruz, R., Ortega‑de-la-Torre, C., Franco‑Topete, R., Franco‑Topete, K., Centeno‑Flores, M. W., Maciel‑Gutiérrez, V. M., Corona‑Rivera, J. R., Armendáriz‑Borunda, J., Bobadilla‑Morales, L."Expression profile of NF-κB regulated genes in sporadic colorectal cancer patients". Oncology Letters 15, no. 5 (2018): 7344-7354. https://doi.org/10.3892/ol.2018.8201