Potential suppressive effects of theophylline on human rectal cancer SW480 cells in vitro by inhibiting YKL-40 expression

  • Authors:
    • Hong Peng
    • Qiang Su
    • Zhong‑Chao Lin
    • Xiu‑Hua Zhu
    • Ming‑Sha Peng
    • Zhen‑Bing Lv
  • View Affiliations

  • Published online on: March 9, 2018     https://doi.org/10.3892/ol.2018.8220
  • Pages: 7403-7408
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Abstract

Chitinase-3-like-1 protein (YKL-40), a member of the mammalian chitinase‑like glycoproteins, serves a key role in the pathogenesis of rectal cancer. The present study examined the antitumor effect of theophylline, a pan‑chitinase inhibitor, in rectal cancer in vitro and investigated the mechanism by which it acted. SW480 cell lines were treated with varying theophylline concentrations (10‑2, 10‑3, 10‑4 and 10‑5 mol/l). An MTT assay was used to observe cell proliferation and identify the optimal theophylline concentration. Western blotting was used to analyze YKL‑40 expression. The cell cycle distribution of SW480 cell lines treated with theophylline was measured by flow cytometry. The angiopoietin‑2 expression level was measured by ELISA. The expression levels of YKL‑40 were evidently decreased in theophylline‑treated SW480 cell lines. The proliferation ofSW480 cells was inhibited following theophylline treatment, which was associated with G1 phase cell cycle arrest and a decrease in the expression of angiopoietin‑2. The mechanism of theophylline action may involve the downregulation of YKL‑40 expression, arrest of the cell cycle at G1 phase and inhibition of angiopoietin‑2 expression. These results provide a rationale for the potential use of anti‑YKL‑40 and anti‑angiogenic strategies in treating rectal cancer.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Peng H, Su Q, Lin ZC, Zhu XH, Peng MS and Lv ZB: Potential suppressive effects of theophylline on human rectal cancer SW480 cells in vitro by inhibiting YKL-40 expression. Oncol Lett 15: 7403-7408, 2018
APA
Peng, H., Su, Q., Lin, Z., Zhu, X., Peng, M., & Lv, Z. (2018). Potential suppressive effects of theophylline on human rectal cancer SW480 cells in vitro by inhibiting YKL-40 expression. Oncology Letters, 15, 7403-7408. https://doi.org/10.3892/ol.2018.8220
MLA
Peng, H., Su, Q., Lin, Z., Zhu, X., Peng, M., Lv, Z."Potential suppressive effects of theophylline on human rectal cancer SW480 cells in vitro by inhibiting YKL-40 expression". Oncology Letters 15.5 (2018): 7403-7408.
Chicago
Peng, H., Su, Q., Lin, Z., Zhu, X., Peng, M., Lv, Z."Potential suppressive effects of theophylline on human rectal cancer SW480 cells in vitro by inhibiting YKL-40 expression". Oncology Letters 15, no. 5 (2018): 7403-7408. https://doi.org/10.3892/ol.2018.8220