Overexpression of CD155 relates to metastasis and invasion in osteosarcoma

Zhuo,Baobiao; Li,Yuan; Gu,Feng; Li,Zhengwei; Sun,Qingzeng; Shi,Yingchun; Shen,Yang; Zhang,Fengfei; Wang,Rong; Wang,Xiaodong

doi:10.3892/ol.2018.8228

Overexpression of CD155 relates to metastasis and invasion in osteosarcoma

Authors:
- Baobiao Zhuo
- Yuan Li
- Feng Gu
- Zhengwei Li
- Qingzeng Sun
- Yingchun Shi
- Yang Shen
- Fengfei Zhang
- Rong Wang
- Xiaodong Wang
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Affiliations: Department of Surgery, Children's Hospital of Soochow University, Suzhou, Jiangsu 215025, P.R. China, Department of Surgery, Xuzhou Children's Hospital, Xuzhou, Jiangsu 221006, P.R. China, Department of Laboratory Medicine, Xuzhou Cancer Hospital, Xuzhou, Jiangsu 221000, P.R. China, Department of Ultrasound, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China
Published online on: March 9, 2018 https://doi.org/10.3892/ol.2018.8228
Pages: 7312-7318

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Abstract

The rapid development of metastatic lesions remains the leading cause of mortality for patients with osteosarcoma. CD155 serves a key role in cancer cell migration, invasion and metastasis. However, the function and mechanism of CD155 has not been explored in osteosarcoma metastasis. In the present study, we found that CD155 was significantly upregulated in lung metastatic tissue and the highly metastatic cell line K7M2‑WT (K7M2) of osteosarcoma. Overexpression of CD155 in K7M2 cells enhanced lung metastasis, while inhibition of CD155 by an anti‑CD155 monoclonal antibody reduced metastasis. Blocking of CD155 also decreased migration and invasion of K7M2 cells in vitro. A western blot analysis revealed that blocking of CD155 inhibits metastasis by downregulating focal adhesion kinase (FAK) and phosphorylated FAK (pFAK) in osteosarcoma. The results revealed that CD155 serves a crucial role in the metastasis of osteosarcoma by regulating FAK and may provide a novel molecular target for therapeutic intervention in metastatic osteosarcoma.

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