miR-486-5p regulates the migration and invasion of colorectal cancer cells through targeting PIK3R1

Zhang,Yuhao; Fu,Jun; Zhang,Zhijin; Qin,Huanlong

doi:10.3892/ol.2018.8233

miR-486-5p regulates the migration and invasion of colorectal cancer cells through targeting PIK3R1

Authors:
- Yuhao Zhang
- Jun Fu
- Zhijin Zhang
- Huanlong Qin
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Affiliations: Department of General Surgery, The Affiliated Shanghai No. 10 People's Hospital, Nanjing Medical University, Shanghai 200072, P.R. China, Department of General Surgery, Shanghai No. 8 People's Hospital, Shanghai 200235, P.R. China
Published online on: March 12, 2018 https://doi.org/10.3892/ol.2018.8233
Pages: 7243-7248

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Abstract

The aim of the present study was to investigate the function of microRNA (miR)-486-5p in colorectal cancer (CRC). Tumor and adjacent normal mucosal tissue samples were collected from patients with CRC. Differences in the expression levels of miR‑486‑5p between tumor tissues and adjacent normal mucosal tissues were examined using reverse transcription-quantitative polymerase chain reaction. The results demonstrated that miR‑486‑5p was significantly decreased in tumor tissues compared with the adjacent normal mucosal tissues. Additionally, in vitro experiments were conducted using SW620 CRC cells. The effects of miR‑486‑5p mimics on cell invasion and cell migration were evaluated using a Transwell assay and a wound‑healing assay, respectively. The results demonstrated that treatment with miR‑486‑5p mimics decreased the migratory and invasive ability of the cells compared with that in the blank and NC control groups, although the underlying molecular mechanisms were not determined. Protein expression levels of phosphatidylinositol 3‑kinase regulatory subunit 1 (PIK3R1), matrix metallopeptidases‑2 and ‑9, and phosphorylated (p)‑AKT were examined using western blot analysis. The results demonstrated that the expression levels of these proteins decreased in response to treatment with miR‑486‑5p mimics in comparison with the blank and NC control groups. Taken together, the findings of the present study indicated that miR‑486‑5p mimics inhibited the progression of CRC by inhibiting the activation of AKT signaling pathway via targeting PIK3R1. Therefore, miR‑486‑5p may be a potential target for CRC treatment.

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