miR-224 enhances invasion and metastasis by targeting HOXD10 in non-small cell lung cancer cells

Li,Shuang; Zhang,Jingang; Zhao,Yunwei; Wang,Fengling; Chen,Ying; Fei,Xiubin

doi:10.3892/ol.2018.8245

miR-224 enhances invasion and metastasis by targeting HOXD10 in non-small cell lung cancer cells

Authors:
- Shuang Li
- Jingang Zhang
- Yunwei Zhao
- Fengling Wang
- Ying Chen
- Xiubin Fei
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Affiliations: Department of Respiratory Medicine, The First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang 154003, P.R. China, Department of Orthopaedic Surgery, Jiamusi Central Hospital, Jiamusi, Heilongjiang 154002, P.R. China, Department of Geriatrics, The First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang 154003, P.R. China, Department of Critical Care Medicine, The First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang 154003, P.R. China
Published online on: March 12, 2018 https://doi.org/10.3892/ol.2018.8245
Pages: 7069-7075
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Increasing number of studies have indicated aberrant microRNA (miRNA) expression could affect normal biological progress in non-small cell lung cancer (NSCLC) cells. This study was performed to evaluate the biologic functions of microRNA-224 (miR-224) in NSCLC. Real-time PCR was performed to evaluate the expression of miR-224 and Homeobox D10 (HOXD10) in NSCLC cell lines and tissues. Transwell assays were performed to investigate the function of miR-224 on NSCLC cell migration and invasion. Moreover, western blotting and luciferase assays were used to investigate HOXD10 as miR-224 downstream targets. miR-224 is increased in NSCLC metastatic tissues and cell lines. Increased miR-224 expression promoted NSCLC cell migration and invasion, while low miR-224 expression suppressed NSCLC cell migration and invasion. Furthermore, HOXD10 was targeted directly by miR-224 in NSCLC cells. Moreover, we found that HOXD10 was a functional target and influenced tumour-inductive functions of miR-224 on progression of NSCLC. These findings suggest that miR-224 may be used in the treatment of NSCLC. Targeting this novel strategy, miR-224/HOXD10 axis may be helpful as promising biomarker and therapeutic method to control NSCLC cell metastasis.

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