Shikonin, vitamin K3 and vitamin K5 inhibit multiple glycolytic enzymes in MCF-7 cells

  • Authors:
    • Jing Chen
    • Xun Hu
    • Jingjie Cui
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  • Published online on: March 13, 2018     https://doi.org/10.3892/ol.2018.8251
  • Pages: 7423-7432
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Abstract

Glycolysis is the most important source of energy for the production of anabolic building blocks in cancer cells. Therefore, glycolytic enzymes are regarded as potential targets for cancer treatment. Previously, naphthaquinones, including shikonin, vitamin K3 and vitamin K5, have been proven to decrease the rate of glycolysis in cancer cells, which is partly due to suppressed pyruvate kinase activity. In the present study, enzymatic assays were performed using MCF‑7 cell lysate in order to screen the profile of glycolytic enzymes in cancer cells inhibited by shikonin, vitamin K3 and vitamin K5, in addition to pyruvate kinase. Results revealed that hexokinase, phosphofructokinase‑1, fructose bisphosphate aldolase, glyceraldehyde‑3‑phosphate dehydrogenase and pyruvate kinase produced in the process of glycolysis were inhibited by shikonin, vitamin K3 and vitamin K5. The results indicated that shikonin, vitamin K3 and vitamin K5 are chemical inhibitors of glycolytic enzymes in cancer cells and have potential uses in translational medical applications.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Chen J, Hu X and Cui J: Shikonin, vitamin K3 and vitamin K5 inhibit multiple glycolytic enzymes in MCF-7 cells. Oncol Lett 15: 7423-7432, 2018
APA
Chen, J., Hu, X., & Cui, J. (2018). Shikonin, vitamin K3 and vitamin K5 inhibit multiple glycolytic enzymes in MCF-7 cells. Oncology Letters, 15, 7423-7432. https://doi.org/10.3892/ol.2018.8251
MLA
Chen, J., Hu, X., Cui, J."Shikonin, vitamin K3 and vitamin K5 inhibit multiple glycolytic enzymes in MCF-7 cells". Oncology Letters 15.5 (2018): 7423-7432.
Chicago
Chen, J., Hu, X., Cui, J."Shikonin, vitamin K3 and vitamin K5 inhibit multiple glycolytic enzymes in MCF-7 cells". Oncology Letters 15, no. 5 (2018): 7423-7432. https://doi.org/10.3892/ol.2018.8251