Open Access

Downregulation of TACC3 inhibits tumor growth and migration in osteosarcoma cells through regulation of the NF-κB signaling pathway

  • Authors:
    • Congran Zhao
    • Xiaofeng He
    • Heng Li
    • Jihui Zhou
    • Xiuying Han
    • Dongjun Wang
    • Guofeng Tian
    • Fuge Sui
  • View Affiliations

  • Published online on: March 14, 2018     https://doi.org/10.3892/ol.2018.8262
  • Pages: 6881-6886
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

TACC3, a member of the transforming acidic coiled-coil protein (TACC) family, is a multifunctional protein that is involved in various biological functions, including proliferation and differentiation of tumor cells, cancer progression and metastasis. The aims of the present study were to examine whether TACC3 expression is associated with the proliferation and migration of osteosarcoma (OS) cells and to investigate the potential underlying molecular mechanisms of TACC3 in OS. First, the levels of mRNA and protein expression in OS cell lines by reverse transcription-quantitative polymerase chain reaction and western blotting, respectively were examined. Second, the effects of TACC3 knockdown and overexpression on the proliferative, migratory and invasive capacities of OS cells were investigated. Finally, western blot analysis was employed to detect the potential mechanism of TACC3 in osteosarcoma. TACC3 expression was significantly increased in osteosarcoma tissues and cell lines, compared to matched controls. The knockdown of TACC3 was able to significantly inhibit the proliferation, migration and invasion of osteosarcoma cells, whereas the overexpression of TACC3 was able to promote cell proliferation and migration. Mechanistically, TACC3 may promote the migration and invasion of osteosarcoma cells via through nuclear factor‑κB signaling. These data suggest that TACC3 has an important part in the progression of osteosarcoma and may serve as a potential target for gene therapy.
View Figures
View References

Related Articles

Journal Cover

May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhao C, He X, Li H, Zhou J, Han X, Wang D, Tian G and Sui F: Downregulation of TACC3 inhibits tumor growth and migration in osteosarcoma cells through regulation of the NF-κB signaling pathway. Oncol Lett 15: 6881-6886, 2018
APA
Zhao, C., He, X., Li, H., Zhou, J., Han, X., Wang, D. ... Sui, F. (2018). Downregulation of TACC3 inhibits tumor growth and migration in osteosarcoma cells through regulation of the NF-κB signaling pathway. Oncology Letters, 15, 6881-6886. https://doi.org/10.3892/ol.2018.8262
MLA
Zhao, C., He, X., Li, H., Zhou, J., Han, X., Wang, D., Tian, G., Sui, F."Downregulation of TACC3 inhibits tumor growth and migration in osteosarcoma cells through regulation of the NF-κB signaling pathway". Oncology Letters 15.5 (2018): 6881-6886.
Chicago
Zhao, C., He, X., Li, H., Zhou, J., Han, X., Wang, D., Tian, G., Sui, F."Downregulation of TACC3 inhibits tumor growth and migration in osteosarcoma cells through regulation of the NF-κB signaling pathway". Oncology Letters 15, no. 5 (2018): 6881-6886. https://doi.org/10.3892/ol.2018.8262