Open Access

Long noncoding AFAP1-antisense RNA 1 is upregulated and promotes tumorigenesis in gastric cancer

  • Authors:
    • Fei Ye
    • Yi Gong
    • Xiangheng Chen
    • Meiying Yu
    • Zhongkun Zuo
    • Dongni Pei
    • Wei Liu
    • Qunwei Wang
    • Jun Zhou
    • Lunxi Duan
    • Leiyi Zhang
    • Xiaojing Li
    • Tenglong Tang
    • Jiangsheng Huang
  • View Affiliations

  • Published online on: March 15, 2018     https://doi.org/10.3892/ol.2018.8266
  • Pages: 7523-7530
  • Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long noncoding RNA serves important roles in gastric cancer (GC). However, the prognostic significance and tumorigenesis effect of AFAP1‑antisense RNA 1 (AS1) in GC remain to be clarified. The present study was conducted in order to determine the expression level of AFAP1‑AS1 by reverse transcription‑quantitative polymerase chain reaction. It was demonstrated that AFAP1‑AS1 expression level was higher in GC tissues in comparison with adjacent tissues. By analyzing 66 GC tissue specimens, AFAP1‑AS1 expression level was found to be markedly associated with tumor size, clinical stage and differentiation. By performing multivariate Cox regression test, AFAP1‑AS1 expression level was confirmed to be an independent factor for poor prognosis in patients with GC. Furthermore, SGC‑7901 and BGC‑823 cells were used for further investigation following transfection of an AFAP1‑AS1 short hairpin RNA lentiviral vector. Knockdown of AFAP1‑AS1 significantly inhibited GC cell proliferation, migration and invasion abilities in vitro. Finally, nude mice experiments confirmed that downregulation of AFAP1‑AS1 in GC cells suppressed tumor growth in vivo. In conclusion, the results of the present study suggested that AFAP1‑AS1 may serve as a valuable prognostic indicator and therapeutic target for GC.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ye F, Gong Y, Chen X, Yu M, Zuo Z, Pei D, Liu W, Wang Q, Zhou J, Duan L, Duan L, et al: Long noncoding AFAP1-antisense RNA 1 is upregulated and promotes tumorigenesis in gastric cancer. Oncol Lett 15: 7523-7530, 2018
APA
Ye, F., Gong, Y., Chen, X., Yu, M., Zuo, Z., Pei, D. ... Huang, J. (2018). Long noncoding AFAP1-antisense RNA 1 is upregulated and promotes tumorigenesis in gastric cancer. Oncology Letters, 15, 7523-7530. https://doi.org/10.3892/ol.2018.8266
MLA
Ye, F., Gong, Y., Chen, X., Yu, M., Zuo, Z., Pei, D., Liu, W., Wang, Q., Zhou, J., Duan, L., Zhang, L., Li, X., Tang, T., Huang, J."Long noncoding AFAP1-antisense RNA 1 is upregulated and promotes tumorigenesis in gastric cancer". Oncology Letters 15.5 (2018): 7523-7530.
Chicago
Ye, F., Gong, Y., Chen, X., Yu, M., Zuo, Z., Pei, D., Liu, W., Wang, Q., Zhou, J., Duan, L., Zhang, L., Li, X., Tang, T., Huang, J."Long noncoding AFAP1-antisense RNA 1 is upregulated and promotes tumorigenesis in gastric cancer". Oncology Letters 15, no. 5 (2018): 7523-7530. https://doi.org/10.3892/ol.2018.8266