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Reduced miR-433 expression is associated with advanced stages and early relapse of colorectal cancer and restored miR-433 expression suppresses the migration, invasion and proliferation of tumor cells in vitro and in nude mice

  • Authors:
    • Jian Zhang
    • Lei Zhang
    • Tong Zhang
    • Xin‑Min Dong
    • Yu Zhu
    • Long‑Hua Chen

  • View Affiliations

  • Published online on: March 15, 2018     https://doi.org/10.3892/ol.2018.8275
  • Pages: 7579-7588

  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The expression of microRNA (miR-433) is altered in various types of human cancer. The present study analyzed the prognostic and biological value of miR‑433 expression in colorectal cancer using reverse transcription-quantitative polymerase chain reaction in 125 colorectal tissue specimens (including a test cohort of 40 cases of paired colorectal cancer and adjacent normal mucosae and a confirmation cohort of 85 cases of stage I‑III colorectal cancer). In vitro and nude mouse xenograft experiments were subsequently used to assess the effects of miR‑433 expression on the regulation of colorectal cancer cell proliferation, adhesion, migration, and invasion. The data indicated that miR‑433 expression was significantly downregulated in colorectal cancer tissues in the test and confirmation patient cohorts and that low miR‑433 expression was associated with advanced tumor stage and early relapse. Furthermore, the restoration of miR‑433 expression was able to significantly inhibit the proliferation of tumor cells by inducing G1‑S cell cycle arrest, suppressing cyclinD1 and CDK4 expression, and markedly inhibited the migratory and invasive capacities of tumor cells in vitro. The restoration of miR‑433 expression or liposome‑based delivery of miR‑433 mimics suppressed the growth of colorectal cancer cell xenografts in nude mice. In conclusion, miR‑433 may be a putative tumor suppressor in colorectal cancer, and the detection of low miR‑433 expression will be investigated in further studies as a putative biomarker for the detection of early relapse in patients with colorectal cancer.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang J, Zhang L, Zhang T, Dong XM, Zhu Y and Chen LH: Reduced miR-433 expression is associated with advanced stages and early relapse of colorectal cancer and restored miR-433 expression suppresses the migration, invasion and proliferation of tumor cells in vitro and in nude mice. Oncol Lett 15: 7579-7588, 2018.
APA
Zhang, J., Zhang, L., Zhang, T., Dong, X., Zhu, Y., & Chen, L. (2018). Reduced miR-433 expression is associated with advanced stages and early relapse of colorectal cancer and restored miR-433 expression suppresses the migration, invasion and proliferation of tumor cells in vitro and in nude mice. Oncology Letters, 15, 7579-7588. https://doi.org/10.3892/ol.2018.8275
MLA
Zhang, J., Zhang, L., Zhang, T., Dong, X., Zhu, Y., Chen, L."Reduced miR-433 expression is associated with advanced stages and early relapse of colorectal cancer and restored miR-433 expression suppresses the migration, invasion and proliferation of tumor cells in vitro and in nude mice". Oncology Letters 15.5 (2018): 7579-7588.
Chicago
Zhang, J., Zhang, L., Zhang, T., Dong, X., Zhu, Y., Chen, L."Reduced miR-433 expression is associated with advanced stages and early relapse of colorectal cancer and restored miR-433 expression suppresses the migration, invasion and proliferation of tumor cells in vitro and in nude mice". Oncology Letters 15, no. 5 (2018): 7579-7588. https://doi.org/10.3892/ol.2018.8275