Tremella polysaccharides inhibit cellular apoptosis and autophagy induced by Pseudomonas aeruginosa lipopolysaccharide in A549 cells through sirtuin 1 activation Retraction in /10.3892/ol.2024.14384

Shi,Xiaolan; Wei,Wenfeng; Wang,Ning

doi:10.3892/ol.2018.8554

Tremella polysaccharides inhibit cellular apoptosis and autophagy induced by Pseudomonas aeruginosa lipopolysaccharide in A549 cells through sirtuin 1 activation

Retraction in: /10.3892/ol.2024.14384

Authors:
- Xiaolan Shi
- Wenfeng Wei
- Ning Wang
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Affiliations: Department of Respiratory and Asthma, Xi'an Children's Hospital, Xi'an, Shanxi 710043, P.R. China, Department of Paediatrics, Xianyang Central Hospital, Xi'an, Shanxi 712000, P.R. China
Published online on: April 23, 2018 https://doi.org/10.3892/ol.2018.8554
Pages: 9609-9616
Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].

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Abstract

In the present study, the role of Tremella polysaccharides in cellular apoptosis and autophagy induced by Pseudomonas aeruginosa lipopolysaccharide (LPS) in human epithelial A549 lung‑cancer cells was investigated. Initially, it was demonstrated that LPS attenuated A549 cell viability in a time‑ and dose‑dependent manner. Furthermore, LPS induced apoptotic cell death and autophagy in A549 cells and increased reactive oxygen species (ROS) production in a time‑dependent manner. In addition, LPS treatment was demonstrated to markedly suppress sirtuin 1 (SIRT1) protein expression in A549 cells. Notably, it was demonstrated that Tremella polysaccharides activate SIRT1, leading to increased p62 expression, decreased p53 acetylation and B‑cell lymphoma 2‑associated X protein expression, and subsequently attenuate LPS‑induced apoptotic cell death and autophagy. The results of the present study demonstrated that Tremella polysaccharides activate SIRT1 and inhibit LPS‑induced ROS production, apoptosis and autophagy. This may have critical implications for the treatment of Pseudomonas aeruginosa infection.

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