miR‑197 is downregulated in cervical carcinogenesis and suppresses cell proliferation and invasion through targeting forkhead box M1

Hu,Qiyan; Du,Ke; Mao,Xiaogang; Ning,Siqing

doi:10.3892/ol.2018.8565

miR‑197 is downregulated in cervical carcinogenesis and suppresses cell proliferation and invasion through targeting forkhead box M1

Authors:
- Qiyan Hu
- Ke Du
- Xiaogang Mao
- Siqing Ning
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Affiliations: Department of Obstetrics and Gynecology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, Hubei 441021, P.R. China
Published online on: April 25, 2018 https://doi.org/10.3892/ol.2018.8565
Pages: 10063-10069

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Abstract

Cervical cancer is the second most common type of cancer in females worldwide. It has been demonstrated that microRNAs (miRs) serve important roles in the occurrence and development of various types of cancer, including cervical cancer. The results of the present study revealed that miR‑197 was downregulated in cervical cancer tissues and cell lines. Restoration of miR‑197 expression significantly inhibited cell viability and invasion of cervical cancer. Additionally, forkhead box M1 (FOXM1) was identified as a direct target gene of miR‑197. Bioinformatic analysis revealed that FOXM1 was a potential target gene of miR‑197. Luciferase reporter assay, reverse transcription‑quantitative polymerase chain reaction and western blot analysis demonstrated that miR‑197 decreased FOXM1 expression through direct binding to its 3'‑untranslated region. Furthermore, the effects of FOXM1 underexpression were comparable with the effects induced by miR‑197 overexpression in cervical cancer cells, suggesting that FOXM1 acted as a downstream effector in miR‑197‑mediated proliferation and invasion of cervical cancer cells. The results of the present study suggested that miR‑197 inhibited growth and metastasis of cervical cancer by directly targeting FOXM1.

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